Details
| Original language | English |
|---|---|
| Title of host publication | Optical Diagnostics and Sensing XXIV |
| Subtitle of host publication | Toward Point-of-Care Diagnostics |
| Editors | Gerard L. Cote, Justin S. Baba |
| Publisher | SPIE |
| Number of pages | 9 |
| ISBN (electronic) | 9781510669598 |
| Publication status | Published - 27 Mar 2024 |
| Event | SPIE BiOS 2024 - San Francisco, United States Duration: 27 Jan 2024 → 1 Feb 2024 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 12850 |
| ISSN (Print) | 1605-7422 |
Abstract
We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.
Keywords
- benchtop imaging device, biochip, fluorescence masking, HSP90a, PMT scanner, Quantum dots
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- Harvard
- Apa
- Vancouver
- BibTeX
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Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics. ed. / Gerard L. Cote; Justin S. Baba. SPIE, 2024. 1285006 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12850).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - A miniaturized test for stress biomarker detection by quantum dots on microarrays
AU - Kishore, Anusha
AU - Varughese, Arun Mathew
AU - Roth, Bernhard
AU - Zeilinger, Carsten
N1 - Funding Information: The authors thank State Bank for Lower Saxony and Saxony-Anhalt, Nord/LB, for receiving funding within the incubator project MSMBest in the framework of the SMINT@Hannover program. B.R. also acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453).
PY - 2024/3/27
Y1 - 2024/3/27
N2 - We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.
AB - We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.
KW - benchtop imaging device
KW - biochip
KW - fluorescence masking
KW - HSP90a
KW - PMT scanner
KW - Quantum dots
UR - http://www.scopus.com/inward/record.url?scp=85190964395&partnerID=8YFLogxK
U2 - 10.1117/12.3002242
DO - 10.1117/12.3002242
M3 - Conference contribution
AN - SCOPUS:85190964395
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Diagnostics and Sensing XXIV
A2 - Cote, Gerard L.
A2 - Baba, Justin S.
PB - SPIE
T2 - SPIE BiOS 2024
Y2 - 27 January 2024 through 1 February 2024
ER -