Details
| Original language | English |
|---|---|
| Article number | 19490 |
| Number of pages | 14 |
| Journal | Scientific reports |
| Volume | 13 |
| Publication status | Published - 9 Nov 2023 |
Abstract
Optogenetics relies on dynamic spatial and temporal control of light to address emerging fundamental and therapeutic questions in cardiac research. In this work, a compact micro-LED array, consisting of 16 × 16 pixels, is incorporated in a widefield fluorescence microscope for controlled light stimulation. We describe the optical design of the system that allows the micro-LED array to fully cover the field of view regardless of the imaging objective used. Various multicellular cardiac models are used in the experiments such as channelrhodopsin-2 expressing aggregates of cardiomyocytes, termed cardiac bodies, and bioartificial cardiac tissues derived from human induced pluripotent stem cells. The pacing efficiencies of the cardiac bodies and bioartificial cardiac tissues were characterized as a function of illumination time, number of switched-on pixels and frequency of stimulation. To demonstrate dynamic stimulation, steering of calcium waves in HL-1 cell monolayer expressing channelrhodopsin-2 was performed by applying different configurations of patterned light. This work shows that micro-LED arrays are powerful light sources for optogenetic control of contraction and calcium waves in cardiac monolayers, multicellular bodies as well as three-dimensional artificial cardiac tissues.
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In: Scientific reports, Vol. 13, 19490, 09.11.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models
AU - Junge, Sebastian
AU - Ricci Signorini, Maria Elena
AU - Al Masri, Masa
AU - Gülink, Jan
AU - Brüning, Heiko
AU - Kasperek, Leon
AU - Szepes, Monika
AU - Bakar, Mine
AU - Gruh, Ina
AU - Heisterkamp, Alexander
AU - Torres-Mapa, Maria Leilani
N1 - Funding Information: We acknowledge the support from the Federal Ministry of Education and Research, Germany (131N4085, Alexander Heisterkamp and Ina Gruh). This study was partly supported by the German Research Foundation, Hearing4all (EXC 2177) (Alexander Heisterkamp) and REBIRTH (EXC 62) (Ina Gruh). This project has also received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 952135 (QubeDot). Maria Leilani Torres-Mapa acknowledges the support of Caroline Herschel Program from the Hochschulbüro für Chancenvielfalt, Leibniz University Hannover.
PY - 2023/11/9
Y1 - 2023/11/9
N2 - Optogenetics relies on dynamic spatial and temporal control of light to address emerging fundamental and therapeutic questions in cardiac research. In this work, a compact micro-LED array, consisting of 16 × 16 pixels, is incorporated in a widefield fluorescence microscope for controlled light stimulation. We describe the optical design of the system that allows the micro-LED array to fully cover the field of view regardless of the imaging objective used. Various multicellular cardiac models are used in the experiments such as channelrhodopsin-2 expressing aggregates of cardiomyocytes, termed cardiac bodies, and bioartificial cardiac tissues derived from human induced pluripotent stem cells. The pacing efficiencies of the cardiac bodies and bioartificial cardiac tissues were characterized as a function of illumination time, number of switched-on pixels and frequency of stimulation. To demonstrate dynamic stimulation, steering of calcium waves in HL-1 cell monolayer expressing channelrhodopsin-2 was performed by applying different configurations of patterned light. This work shows that micro-LED arrays are powerful light sources for optogenetic control of contraction and calcium waves in cardiac monolayers, multicellular bodies as well as three-dimensional artificial cardiac tissues.
AB - Optogenetics relies on dynamic spatial and temporal control of light to address emerging fundamental and therapeutic questions in cardiac research. In this work, a compact micro-LED array, consisting of 16 × 16 pixels, is incorporated in a widefield fluorescence microscope for controlled light stimulation. We describe the optical design of the system that allows the micro-LED array to fully cover the field of view regardless of the imaging objective used. Various multicellular cardiac models are used in the experiments such as channelrhodopsin-2 expressing aggregates of cardiomyocytes, termed cardiac bodies, and bioartificial cardiac tissues derived from human induced pluripotent stem cells. The pacing efficiencies of the cardiac bodies and bioartificial cardiac tissues were characterized as a function of illumination time, number of switched-on pixels and frequency of stimulation. To demonstrate dynamic stimulation, steering of calcium waves in HL-1 cell monolayer expressing channelrhodopsin-2 was performed by applying different configurations of patterned light. This work shows that micro-LED arrays are powerful light sources for optogenetic control of contraction and calcium waves in cardiac monolayers, multicellular bodies as well as three-dimensional artificial cardiac tissues.
UR - http://www.scopus.com/inward/record.url?scp=85176148582&partnerID=8YFLogxK
U2 - 10.1038/s41598-023-46149-1
DO - 10.1038/s41598-023-46149-1
M3 - Article
C2 - 37945622
AN - SCOPUS:85176148582
VL - 13
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
M1 - 19490
ER -