Details
| Original language | English |
|---|---|
| Article number | 313 |
| Journal | Marine drugs |
| Volume | 18 |
| Issue number | 6 |
| Publication status | Published - 14 Jun 2020 |
Abstract
Pseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.
Keywords
- Diatoms, Marine natural products, Mass spectrometry, Metabolomics, Pseudo-nitzschia multistriata, Sexual reproduction
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Drug Discovery
Sustainable Development Goals
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In: Marine drugs, Vol. 18, No. 6, 313, 14.06.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A metabolomics exploration of the sexual phase in the marine diatom pseudo-nitzschia multistriata
AU - Fiorini, Federica
AU - Borgonuovo, Camilla
AU - Ferrante, Maria Immacolata
AU - Brönstrup, Mark
N1 - Funding Information: Funding: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 654008, and was funded in part by the Gordon and Betty Moore Foundation through Grant #7978 to M.I.F. C.B. was supported by an OU SZN PhD fellowship.
PY - 2020/6/14
Y1 - 2020/6/14
N2 - Pseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.
AB - Pseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.
KW - Diatoms
KW - Marine natural products
KW - Mass spectrometry
KW - Metabolomics
KW - Pseudo-nitzschia multistriata
KW - Sexual reproduction
UR - http://www.scopus.com/inward/record.url?scp=85086687019&partnerID=8YFLogxK
U2 - 10.3390/md18060313
DO - 10.3390/md18060313
M3 - Article
C2 - 32545923
AN - SCOPUS:85086687019
VL - 18
JO - Marine drugs
JF - Marine drugs
SN - 1660-3397
IS - 6
M1 - 313
ER -