A labeling study to elucidate the biosynthesis of 4-(4-hydroxyphenyl)-butan-2-one (Raspberry Ketone) by Nidula niveo-tomentosa

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Authors

  • H. Zorn
  • M. Fischer-Zorn
  • R. G. Berger

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Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalApplied and Environmental Microbiology
Volume69
Issue number1
Publication statusPublished - 1 Jan 2003

Abstract

Submerged cells of the basidiomycete Nidula niveo-tomentosa, a microbial producer of 4-(4-hydroxyphenyl)-butan-2-one, were supplemented with 13C-labeled L-phenylalanines and with [1-13C]glucose. Labeled transformation products were detected by a novel method of analyzing stable isotope-labeled metabolites, gas chromatography (GC) coupled to an atomic emission detector, and by GC-mass spectrometry. A benzoate moiety was side chain elongated according to the poly-β-keto scheme. The presence of an acetyl coenzyme A-carboxylase inhibitor shifted the spectrum of products to benzyl compounds. Hence, the fungal pathway differs from the one established for plant tissues.

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A labeling study to elucidate the biosynthesis of 4-(4-hydroxyphenyl)-butan-2-one (Raspberry Ketone) by Nidula niveo-tomentosa. / Zorn, H.; Fischer-Zorn, M.; Berger, R. G.
In: Applied and Environmental Microbiology, Vol. 69, No. 1, 01.01.2003, p. 367-372.

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