Details
| Original language | English |
|---|---|
| Pages (from-to) | 125-135 |
| Number of pages | 11 |
| Journal | Biosensors and Bioelectronics |
| Volume | 5 |
| Issue number | 2 |
| Publication status | Published - 1990 |
Abstract
A novel type of fiber optic biosensor is presented. A miniaturized fiber optic fluorometer is combined with a solid membrane reactor that retains enzymes (dehydrogenases) as well as macromolecular adenine dinucleotide coenzyme derivatives, e.g. PEG (MW 20 000)-N6-(2-aminoethyl)-NAD(H), in the direct vicinity of the sensor tip. The whole system is operated according to the principles of flow-injection analysis (FIA). Three different enzymes (l-lactic dehydrogenase, alcohol dehydrogenase, and glutamic-pyruvic transaminase) were used to demonstrate the feasibility of the novel optical biosensor. Assays for lactate, pyruvate and ethanol were established. In the system described high signal sensitivity and enzyme stability were observed.
Keywords
- biooptrode, enzyme/coenzyme, FIA, fiber optic biosensor, immobilization, macromolecular PEG-NADH, NADH-dependent assays
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 5, No. 2, 1990, p. 125-135.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A Fiber Optic Biosensor based on Fluorometric Detection using Confined Macromolecular Nicotinamide Adenine Dinucleotide Derivatives
AU - Scheper, T.
AU - Bückmann, A. F.
PY - 1990
Y1 - 1990
N2 - A novel type of fiber optic biosensor is presented. A miniaturized fiber optic fluorometer is combined with a solid membrane reactor that retains enzymes (dehydrogenases) as well as macromolecular adenine dinucleotide coenzyme derivatives, e.g. PEG (MW 20 000)-N6-(2-aminoethyl)-NAD(H), in the direct vicinity of the sensor tip. The whole system is operated according to the principles of flow-injection analysis (FIA). Three different enzymes (l-lactic dehydrogenase, alcohol dehydrogenase, and glutamic-pyruvic transaminase) were used to demonstrate the feasibility of the novel optical biosensor. Assays for lactate, pyruvate and ethanol were established. In the system described high signal sensitivity and enzyme stability were observed.
AB - A novel type of fiber optic biosensor is presented. A miniaturized fiber optic fluorometer is combined with a solid membrane reactor that retains enzymes (dehydrogenases) as well as macromolecular adenine dinucleotide coenzyme derivatives, e.g. PEG (MW 20 000)-N6-(2-aminoethyl)-NAD(H), in the direct vicinity of the sensor tip. The whole system is operated according to the principles of flow-injection analysis (FIA). Three different enzymes (l-lactic dehydrogenase, alcohol dehydrogenase, and glutamic-pyruvic transaminase) were used to demonstrate the feasibility of the novel optical biosensor. Assays for lactate, pyruvate and ethanol were established. In the system described high signal sensitivity and enzyme stability were observed.
KW - biooptrode
KW - enzyme/coenzyme
KW - FIA
KW - fiber optic biosensor
KW - immobilization
KW - macromolecular PEG-NADH
KW - NADH-dependent assays
UR - http://www.scopus.com/inward/record.url?scp=0025060175&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(90)80003-V
DO - 10.1016/0956-5663(90)80003-V
M3 - Article
AN - SCOPUS:0025060175
VL - 5
SP - 125
EP - 135
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 2
ER -