Details
| Original language | English |
|---|---|
| Pages (from-to) | C484-C488 |
| Journal | Journal of Food Science |
| Volume | 79 |
| Issue number | 4 |
| Publication status | Published - Apr 2014 |
Abstract
Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma. Practical Application: Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.
Keywords
- 2, 4-dinitrophenylhydrazine, HPLC, Lipid peroxidation, Malondialdehyde, Polyunsaturated fatty acids
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of Food Science, Vol. 79, No. 4, 04.2014, p. C484-C488.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A fast and validated method for the determination of malondialdehyde in fish liver using high-performance liquid chromatography with a photodiode array detector
AU - Faizan, M.
AU - Esatbeyoglu, T.
AU - Bayram, B.
AU - Rimbach, G.
N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/4
Y1 - 2014/4
N2 - Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma. Practical Application: Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.
AB - Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma. Practical Application: Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.
KW - 2
KW - 4-dinitrophenylhydrazine
KW - HPLC
KW - Lipid peroxidation
KW - Malondialdehyde
KW - Polyunsaturated fatty acids
UR - http://www.scopus.com/inward/record.url?scp=84897985001&partnerID=8YFLogxK
U2 - 10.1111/1750-3841.12412
DO - 10.1111/1750-3841.12412
M3 - Article
VL - 79
SP - C484-C488
JO - Journal of Food Science
JF - Journal of Food Science
SN - 0022-1147
IS - 4
ER -