Details
| Translated title of the contribution | Ein thermomorphes stark eutektisches Lösungsmittelmehrphasensystem für biokatalytische Anwendungen |
|---|---|
| Original language | English |
| Article number | e202203823 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 61 |
| Issue number | 31 |
| Early online date | 19 May 2022 |
| Publication status | Published - 25 Jul 2022 |
Abstract
The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger-scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
Keywords
- Aqueous Two-Phase Systems, Biocatalysis, Deep Eutectic Solvents, Enzymes, Thermomorphic Multiphasic Systems
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
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In: Angewandte Chemie - International Edition, Vol. 61, No. 31, e202203823, 25.07.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications
AU - Meyer, Lars-Erik
AU - Andersen, Mads Bruno
AU - Kara, Selin
N1 - Funding Information: S.K. and L.‐E.M. thank the Independent Research Fund Denmark, PHOTOX‐ project, grant No 9063‐00031B, for the grant funding.
PY - 2022/7/25
Y1 - 2022/7/25
N2 - The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger-scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
AB - The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger-scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
KW - Aqueous Two-Phase Systems
KW - Biocatalysis
KW - Deep Eutectic Solvents
KW - Enzymes
KW - Thermomorphic Multiphasic Systems
UR - http://www.scopus.com/inward/record.url?scp=85132139789&partnerID=8YFLogxK
U2 - 10.1002/anie.202203823
DO - 10.1002/anie.202203823
M3 - Article
AN - SCOPUS:85132139789
VL - 61
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 31
M1 - e202203823
ER -