Details
| Original language | English |
|---|---|
| Article number | 371 |
| Journal | Biomolecules |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 28 Feb 2020 |
Abstract
Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.
Keywords
- Bovine pericardium, Decellularization, Porcine pericardium, Surgery, Surgical replacements, Tissue engineering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Biomolecules, Vol. 10, No. 3, 371, 28.02.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia
T2 - New Insights for Surgical Applications
AU - Zouhair, Sabra
AU - Sasso, Eleonora Dal
AU - Tuladhar, Sugat R.
AU - Fidalgo, Catia
AU - Vedovelli, Luca
AU - Filippi, Andrea
AU - Borile, Giulia
AU - Bagno, Andrea
AU - Marchesan, Massimo
AU - De Rossi, Giorgio
AU - Gregori, Dario
AU - Wolkers, Willem F.
AU - Romanato, Filippo
AU - Korossis, Sotirios
AU - Gerosa, Gino
AU - Iop, Laura
N1 - Funding Information: Funding: This research was funded by CA.RI.PA.RO. Foundation for the Padua Heart Project funding, as well as European Union’s Seventh Framework Programme for FP7/2007-2013/ITN-TECAS (agreement: 317512) and Consorzio per la Ricerca Sanitaria (CORIS) (DGR. 1017, 17 July 2018), Veneto Region, Italy for L.I.F.E.L.A.B. Program support.
PY - 2020/2/28
Y1 - 2020/2/28
N2 - Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.
AB - Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.
KW - Bovine pericardium
KW - Decellularization
KW - Porcine pericardium
KW - Surgery
KW - Surgical replacements
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85081001025&partnerID=8YFLogxK
U2 - 10.3390/biom10030371
DO - 10.3390/biom10030371
M3 - Article
C2 - 32121155
AN - SCOPUS:85081001025
VL - 10
JO - Biomolecules
JF - Biomolecules
IS - 3
M1 - 371
ER -