Details
| Original language | English |
|---|---|
| Pages (from-to) | 347-350 |
| Number of pages | 4 |
| Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Volume | 61 |
| Issue number | 5-6 |
| Publication status | Published - 2006 |
Abstract
Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia.
Keywords
- Basidiomycetes, Cell Disruption
ASJC Scopus subject areas
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In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 61, No. 5-6, 2006, p. 347-350.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.
AU - Taskova, Rilka M.
AU - Zorn, Holger
AU - Krings, Ulrich
AU - Bouws, Henning
AU - Berger, Ralf G.
N1 - Funding information: This project is part of the joint initiative “Biolo-gisch aktive Naturstoffe Ð Chemische Diversität” at the University of Hannover. R. Taskova is grateful for the grant by the Deutsche Forschungs-gemeinschaft. We thank Agilent Company for providing us with an Agilent 2100 bioanalyzer.
PY - 2006
Y1 - 2006
N2 - Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia.
AB - Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia.
KW - Basidiomycetes
KW - Cell Disruption
UR - http://www.scopus.com/inward/record.url?scp=33746239560&partnerID=8YFLogxK
U2 - 10.1515/znc-2006-5-608
DO - 10.1515/znc-2006-5-608
M3 - Article
C2 - 16869491
AN - SCOPUS:33746239560
VL - 61
SP - 347
EP - 350
JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
SN - 0939-5075
IS - 5-6
ER -