Details
| Original language | English |
|---|---|
| Pages (from-to) | 225-232 |
| Number of pages | 8 |
| Journal | Journal of microbiological methods |
| Volume | 42 |
| Issue number | 3 |
| Publication status | Published - 19 Oct 2000 |
Abstract
Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment. Copyright (C) 2000 Elsevier Science B.V.
Keywords
- Disintegration, Disruption, Enzymes, Filamentous fungi, Intracellular compounds
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Medicine(all)
- Microbiology (medical)
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of microbiological methods, Vol. 42, No. 3, 19.10.2000, p. 225-232.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A comparative study on the disintegration of filamentous fungi
AU - Taubert, J.
AU - Krings, U.
AU - Berger, R. G.
N1 - Funding information: This work was supported by the Fonds der Chemischen Industrie, Frankfurt.
PY - 2000/10/19
Y1 - 2000/10/19
N2 - Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment. Copyright (C) 2000 Elsevier Science B.V.
AB - Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment. Copyright (C) 2000 Elsevier Science B.V.
KW - Disintegration
KW - Disruption
KW - Enzymes
KW - Filamentous fungi
KW - Intracellular compounds
UR - http://www.scopus.com/inward/record.url?scp=0033815839&partnerID=8YFLogxK
U2 - 10.1016/S0167-7012(00)00194-9
DO - 10.1016/S0167-7012(00)00194-9
M3 - Article
C2 - 11044566
AN - SCOPUS:0033815839
VL - 42
SP - 225
EP - 232
JO - Journal of microbiological methods
JF - Journal of microbiological methods
SN - 0167-7012
IS - 3
ER -