Details
| Original language | English |
|---|---|
| Pages (from-to) | 2846-2851 |
| Number of pages | 6 |
| Journal | Journal of General Virology |
| Volume | 88 |
| Issue number | 10 |
| Publication status | Published - 1 Oct 2007 |
Abstract
The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: Journal of General Virology, Vol. 88, No. 10, 01.10.2007, p. 2846-2851.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection
AU - Dietrich, Christof
AU - Abdallah, Qusai Al
AU - Lintl, Lara
AU - Pietruszka, Agnes
AU - Maiss, Edgar
PY - 2007/10/1
Y1 - 2007/10/1
N2 - The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.
AB - The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.
UR - http://www.scopus.com/inward/record.url?scp=35048886467&partnerID=8YFLogxK
U2 - 10.1099/vir.0.82989-0
DO - 10.1099/vir.0.82989-0
M3 - Article
C2 - 17872539
AN - SCOPUS:35048886467
VL - 88
SP - 2846
EP - 2851
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 10
ER -