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3D-Printed microfluidic device for protein purification in batch chromatography

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Taieb Habib
  • Chantal Brämer
  • Christopher Heuer
  • Jan Ebbecke
  • Sascha Beutel
  • Janina Bahnemann

Research Organisations

External Research Organisations

  • Bielefeld University

Details

Original languageEnglish
Pages (from-to)986-993
Number of pages8
JournalLAB on a chip
Volume22
Issue number5
Publication statusPublished - 27 Jan 2022

Abstract

Modern 3D printers enable not only rapid prototyping, but also high-precision printing-microfluidic devices with channel diameters of just a few micrometres can now be readily assembled using this technology. Such devices offer a myriad of benefits (including miniaturization) that significantly reduce sample and buffer volumes and lead to lower process costs. Although such microfluidic devices are already widely used in the field of biotechnology, there is a lack of research regarding the potential of miniaturization by 3D-printed devices in lab-scale chromatography. In this study, the efficacy of a 3D-printed microfluidic device which provides a substantially lower dead-volume compared to established chromatography systems is demonstrated for batch purification applications. Furthermore, this device enables straightforward integration of various components (such as microfluidic valves and chromatographic units) in an unprecedentedly flexible fashion. Initial proof-of-concept experiments demonstrate successful gradient elution with bovine serum albumin (BSA), and the purification of a pharmaceutically relevant IgG monoclonal antibody (mAb).

ASJC Scopus subject areas

Cite this

3D-Printed microfluidic device for protein purification in batch chromatography. / Habib, Taieb; Brämer, Chantal; Heuer, Christopher et al.
In: LAB on a chip, Vol. 22, No. 5, 27.01.2022, p. 986-993.

Research output: Contribution to journalArticleResearchpeer review

Habib, T, Brämer, C, Heuer, C, Ebbecke, J, Beutel, S & Bahnemann, J 2022, '3D-Printed microfluidic device for protein purification in batch chromatography', LAB on a chip, vol. 22, no. 5, pp. 986-993. https://doi.org/10.1039/d1lc01127h
Habib, T., Brämer, C., Heuer, C., Ebbecke, J., Beutel, S., & Bahnemann, J. (2022). 3D-Printed microfluidic device for protein purification in batch chromatography. LAB on a chip, 22(5), 986-993. https://doi.org/10.1039/d1lc01127h
Habib T, Brämer C, Heuer C, Ebbecke J, Beutel S, Bahnemann J. 3D-Printed microfluidic device for protein purification in batch chromatography. LAB on a chip. 2022 Jan 27;22(5):986-993. doi: 10.1039/d1lc01127h
Habib, Taieb ; Brämer, Chantal ; Heuer, Christopher et al. / 3D-Printed microfluidic device for protein purification in batch chromatography. In: LAB on a chip. 2022 ; Vol. 22, No. 5. pp. 986-993.
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