Details
| Original language | English |
|---|---|
| Article number | 7276 |
| Journal | Scientific reports |
| Volume | 11 |
| Issue number | 1 |
| Publication status | Published - 31 Mar 2021 |
Abstract
With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with kLa values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development.
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In: Scientific reports, Vol. 11, No. 1, 7276, 31.03.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications
T2 - From design to evaluation
AU - Frey, Lasse Jannis
AU - Vorländer, David
AU - Ostsieker, Hendrik
AU - Rasch, Detlev
AU - Lohse, Jan Luca
AU - Breitfeld, Maximilian
AU - Grosch, Jan Hendrik
AU - Wehinger, Gregor D.
AU - Bahnemann, Janina
AU - Krull, Rainer
N1 - Funding information: J.B. gratefully acknowledges the financial support from the Max Buchner Research Foundation of DECHEMA (reference number 3667) and from the German Research Foundation (DFG) via the Emmy Noether Programme (project ID 346772917). The authors would like to thank Anton Enders (Institute of Technical Chemistry, Leibniz University Hannover, Germany) for his support with 3D printing and Sarah Kurakin, Bernhard Müller and Torsten Mayr (Institute of Analytical Chemistry and Food Chemistry, TU Graz, Austria) for providing and spotting the sensor plates.
PY - 2021/3/31
Y1 - 2021/3/31
N2 - With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with kLa values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development.
AB - With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with kLa values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development.
UR - http://www.scopus.com/inward/record.url?scp=85103745972&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-86654-9
DO - 10.1038/s41598-021-86654-9
M3 - Article
C2 - 33790348
AN - SCOPUS:85103745972
VL - 11
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 7276
ER -