Details
| Original language | English |
|---|---|
| Article number | 4421 |
| Number of pages | 16 |
| Journal | Sensors |
| Volume | 20 |
| Issue number | 16 |
| Publication status | Published - 7 Aug 2020 |
Abstract
Electrochemical spectroscopy enables rapid, sensitive, and label‐free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen‐printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio‐)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high‐resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor‐made, experimentspecific systems. In this work, flow cells for SPE‐based electrochemical (bio‐)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamerbased impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D‐printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high‐resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio‐)sensor applications.
Keywords
- Additive manufacturing, Aptasensor, Impedimetric biosensor, Screen‐printed electrodes
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Physics and Astronomy(all)
- Instrumentation
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Sensors, Vol. 20, No. 16, 4421, 07.08.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D-printed flow cells for aptamer-based impedimetric detection of E. coli crooks strain
AU - Siller, Ina G.
AU - Preuss, John Alexander
AU - Urmann, Katharina
AU - Hoffmann, Michael R.
AU - Scheper, Thomas
AU - Bahnemann, Janina
N1 - Funding Information: This research was funded by the Bill and Melinda Gates Foundation (Grant Number OPP1111252) and the German Research Foundation (DFG) via the Emmy Noether program (project ID 346772917). The publication of this article was funded by the Open Access fund of Leibniz Universit?t Hannover. The authors would like to thank the Graduate Academy of the Leibniz University of Hannover for supporting the collaboration and work with the California Institute of Technology in Pasadena,.
PY - 2020/8/7
Y1 - 2020/8/7
N2 - Electrochemical spectroscopy enables rapid, sensitive, and label‐free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen‐printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio‐)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high‐resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor‐made, experimentspecific systems. In this work, flow cells for SPE‐based electrochemical (bio‐)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamerbased impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D‐printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high‐resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio‐)sensor applications.
AB - Electrochemical spectroscopy enables rapid, sensitive, and label‐free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen‐printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio‐)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high‐resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor‐made, experimentspecific systems. In this work, flow cells for SPE‐based electrochemical (bio‐)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamerbased impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D‐printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high‐resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio‐)sensor applications.
KW - Additive manufacturing
KW - Aptasensor
KW - Impedimetric biosensor
KW - Screen‐printed electrodes
UR - http://www.scopus.com/inward/record.url?scp=85089225148&partnerID=8YFLogxK
U2 - 10.3390/s20164421
DO - 10.3390/s20164421
M3 - Article
C2 - 32784793
AN - SCOPUS:85089225148
VL - 20
JO - Sensors
JF - Sensors
SN - 1424-3210
IS - 16
M1 - 4421
ER -