Details
| Original language | English |
|---|---|
| Pages (from-to) | 2422-2437 |
| Number of pages | 16 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 110 |
| Issue number | 11 |
| Early online date | 26 May 2022 |
| Publication status | Published - 16 Sept 2022 |
Abstract
The manufacturing of modern scaffolds with customized geometry and personalization has become possible due to the three-dimensional (3D) printing technique. A novel type of 3D-printed scaffolds for bone tissue regeneration based on poly(ε-caprolactone) (PCL) filled with nanocrystalline cellulose modified by poly(glutamic acid) (PGlu-NCC) has been proposed in this study. The 3D printing set-ups were optimized in order to obtain homogeneous porous scaffolds. Both polymer composites and manufactured 3D scaffolds have demonstrated mechanical properties suitable for a human trabecular bone. Compression moduli were in the range of 334–396 MPa for non-porous PCL and PCL-based composites, and 101–122 MPa for porous scaffolds made of the same materials. In vitro mineralization study with the use of human mesenchymal stem cells (hMSCs) revealed the larger Ca deposits on the surface of PCL/PGlu-NCC composite scaffolds. Implantation of the developed 3D scaffolds into femur of the rabbits was carried out to observe close and delayed effects. The histological analysis showed the lowest content of immune cells and thin fibrous capsule, revealing low toxicity of the PCL/PGlu-NCC scaffolds seeded with rabbit MSCs (rMSCs) to the surrounding tissues. The most pronounced result on the generation of new bone tissue after implantation of PCL/PGlu-NCC + rMSCs scaffolds was detected by both microcomputed tomography and histological analysis. Around 33% and 55% of bone coverage were detected for composite 3D scaffolds with adhered rMSCs after 1 and 3 months of implantation, respectively. This achievement can be a result of synergistic effect of PGlu, which attracts calcium ions, and stem cells with osteogenic potential.
Keywords
- 3D printing, biodegradable composites, bone defects, bone regeneration, cellulose nanocrystals, mesenchymal stem cells, poly(glutamic acid), poly(ε-caprolactone), scaffolds
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 110, No. 11, 16.09.2022, p. 2422-2437.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D-Printed composite scaffolds based on poly(ε-caprolactone) filled with poly(glutamic acid)-modified cellulose nanocrystals for improved bone tissue regeneration
AU - Averianov, Ilia
AU - Stepanova, Mariia
AU - Solomakha, Olga
AU - Gofman, Iosif
AU - Serdobintsev, Mikhail
AU - Blum, Natalya
AU - Kaftuirev, Aleksander
AU - Baulin, Ivan
AU - Nashchekina, Juliya
AU - Lavrentieva, Antonina
AU - Vinogradova, Tatiana
AU - Korzhikov-Vlakh, Viktor
AU - Korzhikova-Vlakh, Evgenia
N1 - Funding Information: The Interdisciplinary Resource Center for Nanotechnology and Centre for X-ray Diffraction Studies of Research Park of St. Petersburg State University are acknowledged for SEM and EDX analysis, and micro-CT, respectively. The authors are very grateful to Mr. Vladimir Kalganov and Mr. Alexander Kulkov for their kind help in processing of SEM/EDX and micro-CT data, respectively. Mr. Ilia Averianov thanks G-RISC program for one-month scholarship supporting his work in Institute of Technical Chemistry, Leibniz University of Hannover (project #M-2019b-6_d).
PY - 2022/9/16
Y1 - 2022/9/16
N2 - The manufacturing of modern scaffolds with customized geometry and personalization has become possible due to the three-dimensional (3D) printing technique. A novel type of 3D-printed scaffolds for bone tissue regeneration based on poly(ε-caprolactone) (PCL) filled with nanocrystalline cellulose modified by poly(glutamic acid) (PGlu-NCC) has been proposed in this study. The 3D printing set-ups were optimized in order to obtain homogeneous porous scaffolds. Both polymer composites and manufactured 3D scaffolds have demonstrated mechanical properties suitable for a human trabecular bone. Compression moduli were in the range of 334–396 MPa for non-porous PCL and PCL-based composites, and 101–122 MPa for porous scaffolds made of the same materials. In vitro mineralization study with the use of human mesenchymal stem cells (hMSCs) revealed the larger Ca deposits on the surface of PCL/PGlu-NCC composite scaffolds. Implantation of the developed 3D scaffolds into femur of the rabbits was carried out to observe close and delayed effects. The histological analysis showed the lowest content of immune cells and thin fibrous capsule, revealing low toxicity of the PCL/PGlu-NCC scaffolds seeded with rabbit MSCs (rMSCs) to the surrounding tissues. The most pronounced result on the generation of new bone tissue after implantation of PCL/PGlu-NCC + rMSCs scaffolds was detected by both microcomputed tomography and histological analysis. Around 33% and 55% of bone coverage were detected for composite 3D scaffolds with adhered rMSCs after 1 and 3 months of implantation, respectively. This achievement can be a result of synergistic effect of PGlu, which attracts calcium ions, and stem cells with osteogenic potential.
AB - The manufacturing of modern scaffolds with customized geometry and personalization has become possible due to the three-dimensional (3D) printing technique. A novel type of 3D-printed scaffolds for bone tissue regeneration based on poly(ε-caprolactone) (PCL) filled with nanocrystalline cellulose modified by poly(glutamic acid) (PGlu-NCC) has been proposed in this study. The 3D printing set-ups were optimized in order to obtain homogeneous porous scaffolds. Both polymer composites and manufactured 3D scaffolds have demonstrated mechanical properties suitable for a human trabecular bone. Compression moduli were in the range of 334–396 MPa for non-porous PCL and PCL-based composites, and 101–122 MPa for porous scaffolds made of the same materials. In vitro mineralization study with the use of human mesenchymal stem cells (hMSCs) revealed the larger Ca deposits on the surface of PCL/PGlu-NCC composite scaffolds. Implantation of the developed 3D scaffolds into femur of the rabbits was carried out to observe close and delayed effects. The histological analysis showed the lowest content of immune cells and thin fibrous capsule, revealing low toxicity of the PCL/PGlu-NCC scaffolds seeded with rabbit MSCs (rMSCs) to the surrounding tissues. The most pronounced result on the generation of new bone tissue after implantation of PCL/PGlu-NCC + rMSCs scaffolds was detected by both microcomputed tomography and histological analysis. Around 33% and 55% of bone coverage were detected for composite 3D scaffolds with adhered rMSCs after 1 and 3 months of implantation, respectively. This achievement can be a result of synergistic effect of PGlu, which attracts calcium ions, and stem cells with osteogenic potential.
KW - 3D printing
KW - biodegradable composites
KW - bone defects
KW - bone regeneration
KW - cellulose nanocrystals
KW - mesenchymal stem cells
KW - poly(glutamic acid)
KW - poly(ε-caprolactone)
KW - scaffolds
UR - http://www.scopus.com/inward/record.url?scp=85130600786&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.35100
DO - 10.1002/jbm.b.35100
M3 - Article
C2 - 35618683
AN - SCOPUS:85130600786
VL - 110
SP - 2422
EP - 2437
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 1552-4973
IS - 11
ER -