Details
| Original language | English |
|---|---|
| Article number | 14584 |
| Journal | Scientific reports |
| Volume | 11 |
| Issue number | 1 |
| Publication status | Published - 16 Jul 2021 |
Abstract
3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig-zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.
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In: Scientific reports, Vol. 11, No. 1, 14584, 16.07.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
AU - Wang, Haoran
AU - Enders, Anton
AU - Preuss, John-Alexander
AU - Bahnemann, Janina
AU - Heisterkamp, Alexander
AU - Torres-Mapa, Maria Leilani
N1 - Funding Information: JB acknowledges the support of the German Research Foundation (DFG) via the Emmy Noether Program, project ID 346772917. MLTM acknowledges the support of Caroline Herschel Program from the Hochschulbüro für Chancenvielfalt, Leibniz University Hannover. This project was partially funded by the German Research Foundation (DFG) excellence clusters H4a (EXC2177) and Rebirth (EXC62). Open Access funding enabled and organized by Projekt DEAL.
PY - 2021/7/16
Y1 - 2021/7/16
N2 - 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig-zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.
AB - 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig-zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.
UR - http://www.scopus.com/inward/record.url?scp=85110702046&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-93205-9
DO - 10.1038/s41598-021-93205-9
M3 - Article
C2 - 34272408
VL - 11
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 14584
ER -