Details
| Original language | English |
|---|---|
| Article number | 035001 |
| Journal | Biomedical Physics and Engineering Express |
| Volume | 2 |
| Issue number | 3 |
| Publication status | Published - 27 Apr 2016 |
| Externally published | Yes |
Abstract
Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.
Keywords
- 3D neural cell culture, Ca imaging, Dissociated hippocampal cultures, Scaffold, Two-photon polymerization
ASJC Scopus subject areas
- Nursing(all)
- General Nursing
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Biomedical Physics and Engineering Express, Vol. 2, No. 3, 035001, 27.04.2016.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function
AU - Timashev, P. S.
AU - Vedunova, M. V.
AU - Guseva, D.
AU - Ponimaskin, E.
AU - Deiwick, A.
AU - Mishchenko, T. A.
AU - Mitroshina, E. V.
AU - Koroleva, A. V.
AU - Pimashkin, A. S.
AU - Mukhina, I. V.
AU - Panchenko, V. Ya
AU - Chichkov, B. N.
AU - Bagratashvili, V. N.
PY - 2016/4/27
Y1 - 2016/4/27
N2 - Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.
AB - Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.
KW - 3D neural cell culture
KW - Ca imaging
KW - Dissociated hippocampal cultures
KW - Scaffold
KW - Two-photon polymerization
UR - http://www.scopus.com/inward/record.url?scp=85007498335&partnerID=8YFLogxK
U2 - 10.1088/2057-1976/2/3/035001
DO - 10.1088/2057-1976/2/3/035001
M3 - Article
AN - SCOPUS:85007498335
VL - 2
JO - Biomedical Physics and Engineering Express
JF - Biomedical Physics and Engineering Express
IS - 3
M1 - 035001
ER -