Details
| Original language | English |
|---|---|
| Pages (from-to) | 2982-2994 |
| Number of pages | 13 |
| Journal | Biomedical Optics Express |
| Volume | 2 |
| Issue number | 11 |
| Publication status | Published - 2011 |
Abstract
Biofilms - communities of microorganisms attached to surfaces - are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far. Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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In: Biomedical Optics Express, Vol. 2, No. 11, 2011, p. 2982-2994.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph
AU - Heidrich, Marko
AU - Kühnel, Mark P.
AU - Kellner, Manuela
AU - Lorbeer, Raoul Amadeus
AU - Lange, Tineke
AU - Winkel, Andreas
AU - Stiesch, Meike
AU - Meyer, Heiko
AU - Heisterkamp, Alexander
PY - 2011
Y1 - 2011
N2 - Biofilms - communities of microorganisms attached to surfaces - are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far. Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.
AB - Biofilms - communities of microorganisms attached to surfaces - are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far. Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.
UR - http://www.scopus.com/inward/record.url?scp=84865811941&partnerID=8YFLogxK
U2 - 10.1364/BOE.2.002982
DO - 10.1364/BOE.2.002982
M3 - Article
AN - SCOPUS:84865811941
VL - 2
SP - 2982
EP - 2994
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 11
ER -