Details
| Original language | English |
|---|---|
| Article number | 153 |
| Journal | Frontiers in Plant Science |
| Volume | 4 |
| Issue number | JUN |
| Publication status | Published - 4 Jun 2013 |
Abstract
Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap http://www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana.
Keywords
- Arabidopsis thaliana, Blue-native, BN/SDS/SDS-PAGE, Mitochondria, NADH dehydrogenase, OXPHOS system, Respiratory chain
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 4, No. JUN, 153, 04.06.2013.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 3D gel map of Arabidopsis complex I
AU - Peters, Katrin
AU - Belt, Katharina
AU - Braun, Hans Peter
PY - 2013/6/4
Y1 - 2013/6/4
N2 - Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap http://www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana.
AB - Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap http://www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana.
KW - Arabidopsis thaliana
KW - Blue-native
KW - BN/SDS/SDS-PAGE
KW - Mitochondria
KW - NADH dehydrogenase
KW - OXPHOS system
KW - Respiratory chain
UR - http://www.scopus.com/inward/record.url?scp=84897061852&partnerID=8YFLogxK
U2 - 10.3389/fpls.2013.00153
DO - 10.3389/fpls.2013.00153
M3 - Article
AN - SCOPUS:84897061852
VL - 4
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - JUN
M1 - 153
ER -