Details
| Original language | English |
|---|---|
| Pages (from-to) | 273-277 |
| Number of pages | 5 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 51 |
| Issue number | 1-3 |
| Publication status | Published - 31 Aug 1998 |
Abstract
The technique of two-dimensional (2D-) fluorescence spectroscopy covers a wide range of excitation and emission wavelengths. Several biogenic fluorophors (proteins, vitamins and coenzymes) can be detected simultaneously. The development of an optical sensor for on-line measurement of 2D-fluorescence spectra at biotechnological processes is described. This sensor is very well suited for in vivo measurements, because it is non-invasive and the on-line data can be performed in-situ using an optical light guide system. This instrumentation was used at cultivations of Saccharomyces cerevisiae to show the potential and also the reliability of 2D-fluorescence spectroscopy for bioprocess monitoring. Changes in cell metabolism (aerobicanaerobic transitions), cell growth, medium composition and also turbidity could be detected.
Keywords
- Bioprocess monitoring, Fluorescence, Optical sensor, Two-dimensional fluorescence spectroscopy
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
- Materials Science(all)
- Materials Chemistry
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In: Sensors and Actuators, B: Chemical, Vol. 51, No. 1-3, 31.08.1998, p. 273-277.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 2-Dimensional fluorescence spectroscopy for on-line bioprocess monitoring
AU - Lindemann, C.
AU - Marose, S.
AU - Nielsen, H. O.
AU - Scheper, T.
N1 - Funding information: The work was supported by a BriteEuram project of the European Comission (BE Project No. BRE-CT94-1005). Partners of this project are DELTA Light and Optics, Denmark; NOVO Nordisk A/S, Denmark, Boehringer Ingelheim Pharma KG, Germany (Dr Karl Thomae GmbH); Optimum, Greece and Institut für Technische Chemie, Universität Hannover, Germany.
PY - 1998/8/31
Y1 - 1998/8/31
N2 - The technique of two-dimensional (2D-) fluorescence spectroscopy covers a wide range of excitation and emission wavelengths. Several biogenic fluorophors (proteins, vitamins and coenzymes) can be detected simultaneously. The development of an optical sensor for on-line measurement of 2D-fluorescence spectra at biotechnological processes is described. This sensor is very well suited for in vivo measurements, because it is non-invasive and the on-line data can be performed in-situ using an optical light guide system. This instrumentation was used at cultivations of Saccharomyces cerevisiae to show the potential and also the reliability of 2D-fluorescence spectroscopy for bioprocess monitoring. Changes in cell metabolism (aerobicanaerobic transitions), cell growth, medium composition and also turbidity could be detected.
AB - The technique of two-dimensional (2D-) fluorescence spectroscopy covers a wide range of excitation and emission wavelengths. Several biogenic fluorophors (proteins, vitamins and coenzymes) can be detected simultaneously. The development of an optical sensor for on-line measurement of 2D-fluorescence spectra at biotechnological processes is described. This sensor is very well suited for in vivo measurements, because it is non-invasive and the on-line data can be performed in-situ using an optical light guide system. This instrumentation was used at cultivations of Saccharomyces cerevisiae to show the potential and also the reliability of 2D-fluorescence spectroscopy for bioprocess monitoring. Changes in cell metabolism (aerobicanaerobic transitions), cell growth, medium composition and also turbidity could be detected.
KW - Bioprocess monitoring
KW - Fluorescence
KW - Optical sensor
KW - Two-dimensional fluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=0347040053&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(98)00195-6
DO - 10.1016/S0925-4005(98)00195-6
M3 - Article
AN - SCOPUS:0347040053
VL - 51
SP - 273
EP - 277
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1-3
ER -