Details
| Original language | English |
|---|---|
| Pages (from-to) | 7613-7620 |
| Number of pages | 8 |
| Journal | Analytical chemistry |
| Volume | 91 |
| Issue number | 12 |
| Early online date | 13 May 2019 |
| Publication status | Published - 18 Jun 2019 |
Abstract
The online hyphenation of chip-based high-performance liquid chromatography (chip-HPLC) with ion mobility spectrometry (IMS) via fully integrated electrospray emitters is introduced. A custom-built drift tube IMS with shifted potentials was developed in order to keep the IMS orifice electrically grounded, allowing for a robust coupling with chip-HPLC. Proof-of-concept studies with the newly developed analytical setup revealed the suitability of IMS as a promising and powerful detection concept for chip-based separation techniques. Comparison of IMS with fluorescence detection and electrospray ionization-mass spectrometry (ESI-MS) allowed a more detailed characterization of the IMS as a new detection method for chip-HPLC. Moreover, the analysis of a mixture consisting of three isobaric antidepressants demonstrated the performance of chip-HPLC/IMS as a miniaturized two-dimensional separation technique.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
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In: Analytical chemistry, Vol. 91, No. 12, 18.06.2019, p. 7613-7620.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - 2D in Seconds
T2 - Coupling of Chip-HPLC with Ion Mobility Spectrometry
AU - Piendl, Sebastian K.
AU - Raddatz, Christian Robert
AU - Hartner, Nora T.
AU - Thoben, Christian
AU - Warias, Rico
AU - Zimmermann, Stefan
AU - Belder, Detlev
N1 - Funding information: This work was funded by the Deutsche Forschungsgemein-schaft (DFG) Project numbers 396430937, 371350234, and FOR 2177.
PY - 2019/6/18
Y1 - 2019/6/18
N2 - The online hyphenation of chip-based high-performance liquid chromatography (chip-HPLC) with ion mobility spectrometry (IMS) via fully integrated electrospray emitters is introduced. A custom-built drift tube IMS with shifted potentials was developed in order to keep the IMS orifice electrically grounded, allowing for a robust coupling with chip-HPLC. Proof-of-concept studies with the newly developed analytical setup revealed the suitability of IMS as a promising and powerful detection concept for chip-based separation techniques. Comparison of IMS with fluorescence detection and electrospray ionization-mass spectrometry (ESI-MS) allowed a more detailed characterization of the IMS as a new detection method for chip-HPLC. Moreover, the analysis of a mixture consisting of three isobaric antidepressants demonstrated the performance of chip-HPLC/IMS as a miniaturized two-dimensional separation technique.
AB - The online hyphenation of chip-based high-performance liquid chromatography (chip-HPLC) with ion mobility spectrometry (IMS) via fully integrated electrospray emitters is introduced. A custom-built drift tube IMS with shifted potentials was developed in order to keep the IMS orifice electrically grounded, allowing for a robust coupling with chip-HPLC. Proof-of-concept studies with the newly developed analytical setup revealed the suitability of IMS as a promising and powerful detection concept for chip-based separation techniques. Comparison of IMS with fluorescence detection and electrospray ionization-mass spectrometry (ESI-MS) allowed a more detailed characterization of the IMS as a new detection method for chip-HPLC. Moreover, the analysis of a mixture consisting of three isobaric antidepressants demonstrated the performance of chip-HPLC/IMS as a miniaturized two-dimensional separation technique.
UR - http://www.scopus.com/inward/record.url?scp=85066412192&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.9b00302
DO - 10.1021/acs.analchem.9b00302
M3 - Article
C2 - 31082255
AN - SCOPUS:85066412192
VL - 91
SP - 7613
EP - 7620
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 12
ER -