Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1979-1983 |
| Seitenumfang | 5 |
| Fachzeitschrift | Journal of Cataract and Refractive Surgery |
| Jahrgang | 35 |
| Ausgabenummer | 11 |
| Publikationsstatus | Veröffentlicht - Nov. 2009 |
| Extern publiziert | Ja |
Abstract
Purpose: To evaluate a new method for visualizing femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Setting: Laser Zentrum Hannover e.V., Hannover, Germany. Method: Lenses removed from porcine eyes were modified ex vivo by femtosecond laser pulses (wavelength 1040 nm, pulse duration 306 femtoseconds, pulse energy 1.0 to 2.5 μJ, repetition rate 100 kHz) to create defined planes at which lens fibers separate. The femtosecond laser pulses were delivered by a 3-dimension (3-D) scanning unit and transmitted by focusing optics (numerical aperture 0.18) into the lens tissue. Lens fiber orientation and femtosecond laser-induced microincisions were examined using a confocal laser scanning microscope (CLSM) based on a Rostock Cornea Module attached to a Heidelberg Retina Tomograph II. Optical sections were analyzed in 3-D using Amira software (version 4.1.1). Results: Normal lens fibers showed a parallel pattern with diameters between 3 μm and 9 μm, depending on scanning location. Microincision visualization showed different cutting effects depending on pulse energy of the femtosecond laser. The effects ranged from altered tissue-scattering properties with all fibers intact to definite fiber separation by a wide gap. Pulse energies that were too high or overlapped too tightly produced an incomplete cutting plane due to extensive microbubble generation. Conclusions: The 3-D CLSM method permitted visualization and analysis of femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Thus, 3-D CLSM may help optimize femtosecond laser-based procedures in the treatment of presbyopia.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Chirurgie
- Medizin (insg.)
- Ophthalmologie
- Neurowissenschaften (insg.)
- Sensorische Systeme
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in: Journal of Cataract and Refractive Surgery, Jahrgang 35, Nr. 11, 11.2009, S. 1979-1983.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Visualization of femtosecond laser pulse-induced microincisions inside crystalline lens tissue
AU - Stachs, Oliver
AU - Schumacher, Silvia
AU - Hovakimyan, Marina
AU - Fromm, Michael
AU - Heisterkamp, Alexander
AU - Lubatschowski, Holger
AU - Guthoff, Rudolf
N1 - Funding information: Supported by BMBF FKZ 13N8709 and 13N8712 and in part by the DFG (Transregio 37, Micro- und Nanosystems in Medicine–Reconstruction of Biological Functions).
PY - 2009/11
Y1 - 2009/11
N2 - Purpose: To evaluate a new method for visualizing femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Setting: Laser Zentrum Hannover e.V., Hannover, Germany. Method: Lenses removed from porcine eyes were modified ex vivo by femtosecond laser pulses (wavelength 1040 nm, pulse duration 306 femtoseconds, pulse energy 1.0 to 2.5 μJ, repetition rate 100 kHz) to create defined planes at which lens fibers separate. The femtosecond laser pulses were delivered by a 3-dimension (3-D) scanning unit and transmitted by focusing optics (numerical aperture 0.18) into the lens tissue. Lens fiber orientation and femtosecond laser-induced microincisions were examined using a confocal laser scanning microscope (CLSM) based on a Rostock Cornea Module attached to a Heidelberg Retina Tomograph II. Optical sections were analyzed in 3-D using Amira software (version 4.1.1). Results: Normal lens fibers showed a parallel pattern with diameters between 3 μm and 9 μm, depending on scanning location. Microincision visualization showed different cutting effects depending on pulse energy of the femtosecond laser. The effects ranged from altered tissue-scattering properties with all fibers intact to definite fiber separation by a wide gap. Pulse energies that were too high or overlapped too tightly produced an incomplete cutting plane due to extensive microbubble generation. Conclusions: The 3-D CLSM method permitted visualization and analysis of femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Thus, 3-D CLSM may help optimize femtosecond laser-based procedures in the treatment of presbyopia.
AB - Purpose: To evaluate a new method for visualizing femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Setting: Laser Zentrum Hannover e.V., Hannover, Germany. Method: Lenses removed from porcine eyes were modified ex vivo by femtosecond laser pulses (wavelength 1040 nm, pulse duration 306 femtoseconds, pulse energy 1.0 to 2.5 μJ, repetition rate 100 kHz) to create defined planes at which lens fibers separate. The femtosecond laser pulses were delivered by a 3-dimension (3-D) scanning unit and transmitted by focusing optics (numerical aperture 0.18) into the lens tissue. Lens fiber orientation and femtosecond laser-induced microincisions were examined using a confocal laser scanning microscope (CLSM) based on a Rostock Cornea Module attached to a Heidelberg Retina Tomograph II. Optical sections were analyzed in 3-D using Amira software (version 4.1.1). Results: Normal lens fibers showed a parallel pattern with diameters between 3 μm and 9 μm, depending on scanning location. Microincision visualization showed different cutting effects depending on pulse energy of the femtosecond laser. The effects ranged from altered tissue-scattering properties with all fibers intact to definite fiber separation by a wide gap. Pulse energies that were too high or overlapped too tightly produced an incomplete cutting plane due to extensive microbubble generation. Conclusions: The 3-D CLSM method permitted visualization and analysis of femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Thus, 3-D CLSM may help optimize femtosecond laser-based procedures in the treatment of presbyopia.
UR - http://www.scopus.com/inward/record.url?scp=72049128576&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2009.06.019
DO - 10.1016/j.jcrs.2009.06.019
M3 - Article
C2 - 19878832
AN - SCOPUS:72049128576
VL - 35
SP - 1979
EP - 1983
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
SN - 0886-3350
IS - 11
ER -