Variations and taxonomic status of begomoviruses causing severe epidemics of cassava mosaic disease in Kenya, Uganda, and Democratic Republic of the Congo

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Hassan Karakacha Were
  • Stephan Winter
  • Edgar Maiss

Organisationseinheiten

Externe Organisationen

  • Kyushu University
  • Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)243-248
Seitenumfang6
FachzeitschriftJournal of general plant pathology
Jahrgang70
Ausgabenummer4
PublikationsstatusVeröffentlicht - Aug. 2004

Abstract

The molecular variability in the DNA-A of cassava-begomoviruses, East African cassava mosaic virus (EACMV), East African cassava mosaic virus - Uganda variant (EACMV-UG), African cassava mosaic virus (ACMV), and East African cassava mosaic Zanzibar virus (EACMZV) in Kenya, Uganda, and Democratic Republic of the Congo (DRC) was investigated. Most samples from western Kenya, Uganda, and eastern DRC contained EACMV-UG. Fewer than half of the samples had a mixed infection of ACMV and EACMV-UG, and a small percentage had only ACMV infections. EACMV and EACMZV were the only begomoviruses detected in samples from the Kenyan coast. The coat protein gene nucleotide (nt) and the deduced amino acid sequence analyses revealed a high degree of sequence identity within each virus type and that EACMV-UG was most related to ACMV. However, analysis of the retrieved complete DNA-A (2781-2801 nt) sequences of selected virus types revealed that EACMV-UG DNA-A share more than 90% identity with EACMV and less than 80% with ACMV, confirming that the virus is a strain of EACMV.

ASJC Scopus Sachgebiete

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Variations and taxonomic status of begomoviruses causing severe epidemics of cassava mosaic disease in Kenya, Uganda, and Democratic Republic of the Congo. / Were, Hassan Karakacha; Winter, Stephan; Maiss, Edgar.
in: Journal of general plant pathology, Jahrgang 70, Nr. 4, 08.2004, S. 243-248.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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abstract = "The molecular variability in the DNA-A of cassava-begomoviruses, East African cassava mosaic virus (EACMV), East African cassava mosaic virus - Uganda variant (EACMV-UG), African cassava mosaic virus (ACMV), and East African cassava mosaic Zanzibar virus (EACMZV) in Kenya, Uganda, and Democratic Republic of the Congo (DRC) was investigated. Most samples from western Kenya, Uganda, and eastern DRC contained EACMV-UG. Fewer than half of the samples had a mixed infection of ACMV and EACMV-UG, and a small percentage had only ACMV infections. EACMV and EACMZV were the only begomoviruses detected in samples from the Kenyan coast. The coat protein gene nucleotide (nt) and the deduced amino acid sequence analyses revealed a high degree of sequence identity within each virus type and that EACMV-UG was most related to ACMV. However, analysis of the retrieved complete DNA-A (2781-2801 nt) sequences of selected virus types revealed that EACMV-UG DNA-A share more than 90% identity with EACMV and less than 80% with ACMV, confirming that the virus is a strain of EACMV.",
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AU - Winter, Stephan

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N1 - Funding Information: Acknowledgments We sincerely thank Prof. Dr. Y. Takanami and Dr. Lina Katul for their useful discussions and advice. We also thank Prof. Dr. P.O. Ayiecho for his guidance during sample collection in Kenya and Mr. Pierre E. Bolonge for providing infected cassava samples from the DRC. This work was funded in part by the German Academic Exchange Service (DAAD).

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