TY - JOUR

T1 - Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana

AU - Carrie, Chris

AU - Murcha, Monika W.

AU - Kuehn, Kristina

AU - Duncan, Owen

AU - Barthet, Michelle

AU - Smith, Penelope M.

AU - Eubel, Holger

AU - Meyer, Etienne

AU - Day, David A.

AU - Millar, A. Harvey

AU - Whelan, James

N1 - Funding information: This work was supported by an Australian Research Council Grant DP0664692, ARC Australian Postdoctoral Fellowships to M.W.M. and H.E., and an ARC Australian Professorial Fellowship to A.H.M.

PY - 2008/9/3

Y1 - 2008/9/3

N2 - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.

AB - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.

KW - Alternative NAD(P)H dehydrogenase

KW - Chloroplast

KW - Dual targeting

KW - Green fluorescent protein

KW - Mitochondria

KW - Peroxisome

UR - http://www.scopus.com/inward/record.url?scp=49649083497&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.07.061

DO - 10.1016/j.febslet.2008.07.061

M3 - Article

C2 - 18703057

AN - SCOPUS:49649083497

VL - 582

SP - 3073

EP - 3079

JO - FEBS letters

JF - FEBS letters

SN - 0014-5793

IS - 20

ER -