Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Chris Carrie
  • Monika W. Murcha
  • Kristina Kuehn
  • Owen Duncan
  • Michelle Barthet
  • Penelope M. Smith
  • Holger Eubel
  • Etienne Meyer
  • David A. Day
  • A. Harvey Millar
  • James Whelan

Externe Organisationen

  • ARC Centre of Excellence in Plant Energy Biology
  • Universität Sydney
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Details

OriginalspracheEnglisch
Seiten (von - bis)3073-3079
Seitenumfang7
FachzeitschriftFEBS letters
Jahrgang582
Ausgabenummer20
PublikationsstatusVeröffentlicht - 3 Sept. 2008
Extern publiziertJa

Abstract

We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.

ASJC Scopus Sachgebiete

Zitieren

Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana. / Carrie, Chris; Murcha, Monika W.; Kuehn, Kristina et al.
in: FEBS letters, Jahrgang 582, Nr. 20, 03.09.2008, S. 3073-3079.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Carrie, C, Murcha, MW, Kuehn, K, Duncan, O, Barthet, M, Smith, PM, Eubel, H, Meyer, E, Day, DA, Millar, AH & Whelan, J 2008, 'Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana', FEBS letters, Jg. 582, Nr. 20, S. 3073-3079. https://doi.org/10.1016/j.febslet.2008.07.061
Carrie, C., Murcha, M. W., Kuehn, K., Duncan, O., Barthet, M., Smith, P. M., Eubel, H., Meyer, E., Day, D. A., Millar, A. H., & Whelan, J. (2008). Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana. FEBS letters, 582(20), 3073-3079. https://doi.org/10.1016/j.febslet.2008.07.061
Carrie C, Murcha MW, Kuehn K, Duncan O, Barthet M, Smith PM et al. Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana. FEBS letters. 2008 Sep 3;582(20):3073-3079. doi: 10.1016/j.febslet.2008.07.061
Carrie, Chris ; Murcha, Monika W. ; Kuehn, Kristina et al. / Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana. in: FEBS letters. 2008 ; Jahrgang 582, Nr. 20. S. 3073-3079.
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abstract = "We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.",
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AU - Carrie, Chris

AU - Murcha, Monika W.

AU - Kuehn, Kristina

AU - Duncan, Owen

AU - Barthet, Michelle

AU - Smith, Penelope M.

AU - Eubel, Holger

AU - Meyer, Etienne

AU - Day, David A.

AU - Millar, A. Harvey

AU - Whelan, James

N1 - Funding information: This work was supported by an Australian Research Council Grant DP0664692, ARC Australian Postdoctoral Fellowships to M.W.M. and H.E., and an ARC Australian Professorial Fellowship to A.H.M.

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Y1 - 2008/9/3

N2 - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.

AB - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.

KW - Alternative NAD(P)H dehydrogenase

KW - Chloroplast

KW - Dual targeting

KW - Green fluorescent protein

KW - Mitochondria

KW - Peroxisome

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JF - FEBS letters

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