Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 345-356 |
| Seitenumfang | 12 |
| Fachzeitschrift | Journal of biotechnology |
| Jahrgang | 31 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - Dez. 1993 |
| Extern publiziert | Ja |
Abstract
In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and β-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Journal of biotechnology, Jahrgang 31, Nr. 3, 12.1993, S. 345-356.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Two FIA-based biosensor systems studied for bioprocess monitoring
AU - Scheper, Th
AU - Brandes, W.
AU - Maschke, H.
AU - Plötz, F.
AU - Müller, C.
N1 - Funding information: The authors gratefully acknowledge the financial support of the Deutsche Forschungsgemeinschaft.
PY - 1993/12
Y1 - 1993/12
N2 - In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and β-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.
AB - In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and β-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.
KW - Biosensor
KW - Calorimetry
KW - FIA
KW - Flow injection analysis
KW - Immunoanalysis
KW - Optode
UR - http://www.scopus.com/inward/record.url?scp=0027746159&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(93)90079-3
DO - 10.1016/0168-1656(93)90079-3
M3 - Article
C2 - 7764442
AN - SCOPUS:0027746159
VL - 31
SP - 345
EP - 356
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 3
ER -