TY - JOUR

T1 - Transferrin-decorated niosomes with integrated inp/zns quantum dots and magnetic iron oxide nanoparticles

T2 - Dual targeting and imaging of glioma

AU - Seleci, Didem Ag

AU - Maurer, Viktor

AU - Barlas, Firat Baris

AU - Porsiel, Julian Cedric

AU - Temel, Bilal

AU - Ceylan, Elcin

AU - Timur, Suna

AU - Stahl, Frank

AU - Scheper, Thomas

AU - Garnweitner, Georg

N1 - Funding Information: Funding: This research was partially funded by Niedersächsisches Ministerium für Wissenschaft und Kultur through the “Quantum-and Nano-Metrology (QUANOMET)” initiative (ZN3245) within the project NP-1. Moreover, we acknowledge support from the German Research Foundation and the Open Access Publication Funds of Technische Universität Braunschweig.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEG-NIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.

AB - The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEG-NIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.

KW - Glioma imaging

KW - Iron oxide nanoparticles

KW - Multifunctional niosomes

KW - Quantum dots

UR - http://www.scopus.com/inward/record.url?scp=85104753811&partnerID=8YFLogxK

U2 - 10.3390/ijms22094556

DO - 10.3390/ijms22094556

M3 - Article

C2 - 33925347

AN - SCOPUS:85104753811

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 9

M1 - 4556

ER -