Transcriptome analysis using next-generation sequencing

Publikation: Beitrag in FachzeitschriftÜbersichtsarbeitForschungPeer-Review

Autorschaft

  • Kai-Oliver Mutz
  • Alexandra Heilkenbrinker
  • Maren Lönne
  • Johanna-Gabriela Walter
  • Frank Stahl

Organisationseinheiten

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Details

OriginalspracheEnglisch
Seiten (von - bis)22-30
Seitenumfang9
FachzeitschriftCurrent opinion in biotechnology
Jahrgang24
Ausgabenummer1
PublikationsstatusVeröffentlicht - Feb. 2013

Abstract

Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world.

Zitieren

Transcriptome analysis using next-generation sequencing. / Mutz, Kai-Oliver; Heilkenbrinker, Alexandra; Lönne, Maren et al.
in: Current opinion in biotechnology, Jahrgang 24, Nr. 1, 02.2013, S. 22-30.

Publikation: Beitrag in FachzeitschriftÜbersichtsarbeitForschungPeer-Review

Mutz KO, Heilkenbrinker A, Lönne M, Walter JG, Stahl F. Transcriptome analysis using next-generation sequencing. Current opinion in biotechnology. 2013 Feb;24(1):22-30. doi: 10.1016/j.copbio.2012.09.004
Mutz, Kai-Oliver ; Heilkenbrinker, Alexandra ; Lönne, Maren et al. / Transcriptome analysis using next-generation sequencing. in: Current opinion in biotechnology. 2013 ; Jahrgang 24, Nr. 1. S. 22-30.
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AU - Mutz, Kai-Oliver

AU - Heilkenbrinker, Alexandra

AU - Lönne, Maren

AU - Walter, Johanna-Gabriela

AU - Stahl, Frank

N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.

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N2 - Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world.

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KW - Gene Expression Profiling/methods

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KW - Humans

KW - Microarray Analysis

KW - RNA/analysis

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KW - Transcriptome/genetics

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JO - Current opinion in biotechnology

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