Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Ophthalmic Technologies XX |
| Publikationsstatus | Veröffentlicht - 2 März 2010 |
| Extern publiziert | Ja |
| Veranstaltung | Ophthalmic Technologies XX - San Francisco, CA, USA / Vereinigte Staaten Dauer: 23 Jan. 2010 → 25 Jan. 2010 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 7550 |
| ISSN (Print) | 1605-7422 |
Abstract
Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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- BibTex
- RIS
Ophthalmic Technologies XX. 2010. 755002 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 7550).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Three-dimensional multimodal microscopy of rabbit cornea after cross-linking treatment
AU - Krüger, Alexander
AU - Hovakimyan, Marina
AU - Ramírez, D. F.
AU - Lorbeer, R. A.
AU - Kröger, Matthias
AU - Stachs, Oliver
AU - Wree, Andreas
AU - Guthoff, Rudolf Friedrich
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2010/3/2
Y1 - 2010/3/2
N2 - Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
AB - Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
KW - Confocal laser scanning microscopy
KW - Cornea
KW - Cross-linking
KW - Keratoconus
KW - Nonlinear microscopy
KW - Second harmonic imaging
KW - Two photon excited fluorescence
UR - http://www.scopus.com/inward/record.url?scp=79953067159&partnerID=8YFLogxK
U2 - 10.1117/12.842282
DO - 10.1117/12.842282
M3 - Conference contribution
AN - SCOPUS:79953067159
SN - 9780819479464
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Ophthalmic Technologies XX
T2 - Ophthalmic Technologies XX
Y2 - 23 January 2010 through 25 January 2010
ER -