Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 755-769 |
| Seitenumfang | 15 |
| Fachzeitschrift | New Phytologist |
| Jahrgang | 193 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 1 Feb. 2012 |
Abstract
• The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. • We generated a set of 25906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. • We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. • Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Physiologie
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: New Phytologist, Jahrgang 193, Nr. 3, 01.02.2012, S. 755-769.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont
AU - Tisserant, E.
AU - Kohler, A.
AU - Dozolme-Seddas, P.
AU - Balestrini, R.
AU - Benabdellah, K.
AU - Colard, A.
AU - Croll, D.
AU - da Silva, C.
AU - Gomez, S. K.
AU - Koul, R.
AU - Ferrol, N.
AU - Fiorilli, V.
AU - Formey, D.
AU - Franken, P. H.
AU - Helber, N.
AU - Hijri, M.
AU - Lanfranco, L.
AU - Lindquist, E.
AU - Liu, Y.
AU - Malbreil, M.
AU - Morin, E.
AU - Poulain, J.
AU - Shapiro, H.
AU - van Tuinen, D.
AU - Waschke, A.
AU - Azcón-Aguilar, C.
AU - Bécard, G.
AU - Bonfante, P.
AU - Harrison, M. J.
AU - Küster, H.
AU - Lammers, P.
AU - Paszkowski, U.
AU - Requena, N.
AU - Rensing, S. A.
AU - Roux, C.
AU - Sanders, I. R.
AU - Shachar-Hill, Y.
AU - Tuskan, G.
AU - Young, J. P.W.
AU - Gianinazzi-Pearson, V.
AU - Martin, F.
PY - 2012/2/1
Y1 - 2012/2/1
N2 - • The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. • We generated a set of 25906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. • We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. • Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.
AB - • The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. • We generated a set of 25906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. • We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. • Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.
KW - Glomeromycota
KW - Glomus
KW - Meiosis and recombination genes
KW - Mycorrhiza
KW - Small secreted proteins
KW - Symbiosis
KW - Transcript profiling
UR - http://www.scopus.com/inward/record.url?scp=84855792250&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.2011.03948.x
DO - 10.1111/j.1469-8137.2011.03948.x
M3 - Article
C2 - 22092242
AN - SCOPUS:84855792250
VL - 193
SP - 755
EP - 769
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 3
ER -