Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 4832-4838 |
| Seitenumfang | 7 |
| Fachzeitschrift | PROTEOMICS |
| Jahrgang | 5 |
| Ausgabenummer | 18 |
| Publikationsstatus | Veröffentlicht - 12 Dez. 2005 |
Abstract
Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
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in: PROTEOMICS, Jahrgang 5, Nr. 18, 12.12.2005, S. 4832-4838.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The secretome of Pleurotus sapidus
AU - Zorn, Holger
AU - Peters, Thilo
AU - Nimtz, Manfred
AU - Berger, Ralf G.
PY - 2005/12/12
Y1 - 2005/12/12
N2 - Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
AB - Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
KW - 2-D electrophoresis
KW - Basidiomycete
KW - Extracellular enzymes
KW - Fungi
UR - http://www.scopus.com/inward/record.url?scp=28944455667&partnerID=8YFLogxK
U2 - 10.1002/pmic.200500015
DO - 10.1002/pmic.200500015
M3 - Article
C2 - 16281184
AN - SCOPUS:28944455667
VL - 5
SP - 4832
EP - 4838
JO - PROTEOMICS
JF - PROTEOMICS
SN - 1615-9853
IS - 18
ER -