Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 122-125 |
| Seitenumfang | 4 |
| Fachzeitschrift | Virus research |
| Jahrgang | 127 |
| Ausgabenummer | 1 |
| Frühes Online-Datum | 4 Mai 2007 |
| Publikationsstatus | Veröffentlicht - 1 Juli 2007 |
Abstract
The Potato virus Y (PVY) cDNA full-length clone created by Jakab et al. [Jakab, G., Droz, E., Brigneti, G., Baulcombe, D., Malnoë, P., 1997. Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J. Gen. Virol. 78, 3141-3145] was stabilized by inserting three introns into putatively toxic genes. Using this clone, hybrid viruses were constructed by in vitro recombination. The PVY-N/NTN and PVY-N/O chimeras carried the 3′ end of NIb, the whole CP and 3′UTR region of PVYNTN and PVYO, respectively, in a PVYN genetic background. The clones proved to be stable after several passages by re-sequencing the exchanged region. Both hybrid viruses showed reduced infectivity in particle bombardment experiments, but they were suitable for further mechanical plant inoculation. In five of the six host plant species, inoculated with the two chimeras and three parental strains, the chimeras produced similar symptoms to those of PVYN. By contrast, Physalis floridana reacted with different pattern of symptoms. In this species, the symptoms caused by the N/O hybrid were similar to those of the 3′NIb-CP-donating PVYO strain, and not to those of the background (PVYN). The results suggest that symptom determinants may be different even between strains of the same virus species in a particular host.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Virologie
- Medizin (insg.)
- Infektionskrankheiten
- Biochemie, Genetik und Molekularbiologie (insg.)
- Krebsforschung
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in: Virus research, Jahrgang 127, Nr. 1, 01.07.2007, S. 122-125.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The role of the coat protein region in symptom formation on Physalis floridana varies between PVY strains
AU - Bukovinszki, Ágnes
AU - Götz, Reinhard
AU - Johansen, Elisabeth
AU - Maiss, Edgar
AU - Balázs, Ervin
N1 - Funding Information: We thank Brigitta Dudás and Barbara Harasztos for critical reading and editing the manuscript. Á. Bukovinszki was supported by the German Academic Exchange Service (DAAD). The assistance of staff members of IPP Hannover is greatly appreciated. This work was supported by grants TS 44778 and NI 61023 from the Hungarian Scientific Research Fund (OTKA).
PY - 2007/7/1
Y1 - 2007/7/1
N2 - The Potato virus Y (PVY) cDNA full-length clone created by Jakab et al. [Jakab, G., Droz, E., Brigneti, G., Baulcombe, D., Malnoë, P., 1997. Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J. Gen. Virol. 78, 3141-3145] was stabilized by inserting three introns into putatively toxic genes. Using this clone, hybrid viruses were constructed by in vitro recombination. The PVY-N/NTN and PVY-N/O chimeras carried the 3′ end of NIb, the whole CP and 3′UTR region of PVYNTN and PVYO, respectively, in a PVYN genetic background. The clones proved to be stable after several passages by re-sequencing the exchanged region. Both hybrid viruses showed reduced infectivity in particle bombardment experiments, but they were suitable for further mechanical plant inoculation. In five of the six host plant species, inoculated with the two chimeras and three parental strains, the chimeras produced similar symptoms to those of PVYN. By contrast, Physalis floridana reacted with different pattern of symptoms. In this species, the symptoms caused by the N/O hybrid were similar to those of the 3′NIb-CP-donating PVYO strain, and not to those of the background (PVYN). The results suggest that symptom determinants may be different even between strains of the same virus species in a particular host.
AB - The Potato virus Y (PVY) cDNA full-length clone created by Jakab et al. [Jakab, G., Droz, E., Brigneti, G., Baulcombe, D., Malnoë, P., 1997. Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J. Gen. Virol. 78, 3141-3145] was stabilized by inserting three introns into putatively toxic genes. Using this clone, hybrid viruses were constructed by in vitro recombination. The PVY-N/NTN and PVY-N/O chimeras carried the 3′ end of NIb, the whole CP and 3′UTR region of PVYNTN and PVYO, respectively, in a PVYN genetic background. The clones proved to be stable after several passages by re-sequencing the exchanged region. Both hybrid viruses showed reduced infectivity in particle bombardment experiments, but they were suitable for further mechanical plant inoculation. In five of the six host plant species, inoculated with the two chimeras and three parental strains, the chimeras produced similar symptoms to those of PVYN. By contrast, Physalis floridana reacted with different pattern of symptoms. In this species, the symptoms caused by the N/O hybrid were similar to those of the 3′NIb-CP-donating PVYO strain, and not to those of the background (PVYN). The results suggest that symptom determinants may be different even between strains of the same virus species in a particular host.
KW - Hybrid virus
KW - Infectious cDNA
KW - Physalis floridana
KW - Potato virus Y
KW - Symptom determinants
UR - http://www.scopus.com/inward/record.url?scp=34249021716&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2007.03.023
DO - 10.1016/j.virusres.2007.03.023
M3 - Article
C2 - 17482305
AN - SCOPUS:34249021716
VL - 127
SP - 122
EP - 125
JO - Virus research
JF - Virus research
SN - 0168-1702
IS - 1
ER -