TY - JOUR

T1 - The role of amino acid metabolism during abiotic stress release

AU - Batista-Silva, Willian

AU - Heinemann, Björn

AU - Rugen, Nils

AU - Nunes-Nesi, Adriano

AU - Araújo, Wagner L.

AU - Braun, Hans Peter

AU - Hildebrandt, Tatjana M.

N1 - Funding information: We thank Christa Ruppelt for excellent technical assistance in performing the root toxicity tests. This work was supported by the binational science funding programme “PROBRAL” of the Deutsche Akademische Austauschdienst (DAAD; funds provided by the Bundesministerium für Bildung und Forschung—BMBF) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Refs: Project?ID PROBRAL #423/14. Research fellowships granted by the National Council for Scientific and Technological Development (CNPq?Brazil) to A.N.N. and W.L.A. are gratefully acknowledged. We thank Christa Ruppelt for excellent technical assistance in performing the root toxicity tests. This work was supported by the binational science funding programme ?PROBRAL? of the Deutsche Akademische Austauschdienst (DAAD; funds provided by the Bundesministerium f?r Bildung und Forschung?BMBF) and the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES), Refs: Project-ID PROBRAL #423/14. Research fellowships granted by the National Council for Scientific and Technological Development (CNPq-Brazil) to A.N.N. and W.L.A. are gratefully acknowledged.

PY - 2019/4/12

Y1 - 2019/4/12

N2 - Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.

AB - Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.

KW - Arabidopsis thaliana

KW - drought

KW - hydroponic culture

KW - proteomics

KW - salinity

KW - Proline/metabolism

KW - Oxidative Stress

KW - Lysine/metabolism

KW - Plant Leaves/metabolism

KW - Stress, Physiological

KW - Amino Acids/metabolism

KW - Dehydration

KW - Proteome/metabolism

KW - Arabidopsis/physiology

KW - Salt Stress

KW - Arabidopsis Proteins/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85061281882&partnerID=8YFLogxK

U2 - 10.1111/pce.13518

DO - 10.1111/pce.13518

M3 - Article

C2 - 30632176

AN - SCOPUS:85061281882

VL - 42

SP - 1630

EP - 1644

JO - Plant Cell and Environment

JF - Plant Cell and Environment

SN - 0140-7791

IS - 5

ER -