TY - JOUR

T1 - The Osteogenic and Tenogenic Differentiation Potential of C3H10T1/2 (Mesenchymal Stem Cell Model) Cultured on PCL/PLA Electrospun Scaffolds in the Absence of Specific Differentiation Medium

AU - Glasmacher, Birgit

AU - Müller, Marc

AU - Baudequin, Timothée

AU - Gaut, Ludovic

AU - Huepkes, Angela

AU - Duprez, Delphine

AU - Bedoui, Fahmi

AU - Legallais, Cécile

N1 - Funding information: Acknowledgments: The authors would like to thank Guy Schlatter and Corinne Wittmer (ICPEES Strasbourg) for the pure PCL scaffolds and fruitful discussions. This research was supported by the Picardy region, the Equipex FIGURES, Convergence Sorbonne Universités programme (MecaMusTen project) and the Fondation pour la Recherche Médicale (FRM; grant DEQ20140329500). This project was cofinanced by the European Union and the European Regional Development Fund. This work was carried out and funded in the framework of the Labex MS2T. It was supported by the French government, through the program “Investments for the Future” managed by the National Agency for Research (Reference ANR-11-IDEX-0004-02). Timothée Baudequin acknowledges the financial support of the CNRS and the Collegium INSIS-UTC. Timothée Baudequin and Marc Mueller acknowledge the financial support of the yESAO exchange program. Angela Huepkes acknowledges the financial support of the Erasmus program.

PY - 2017/12/4

Y1 - 2017/12/4

N2 - The differentiation potential of mesenchymal stem cells (MSC) has been extensively tested on electrospun scaffolds. However, this potential is often assessed with lineage-specific medium, making it difficult to interpret the real contribution of the properties of the scaffold in the cell response. In this study, we analyzed the ability of different polycaprolactone/polylactic acid PCL/PLA electrospun scaffolds (pure or blended compositions, random or aligned fibers, various fiber diameters) to drive MSC towards bone or tendon lineages in the absence of specific differentiation medium. C3H10T1/2 cells (a mesenchymal stem cell model) were cultured on scaffolds for 96 h without differentiation factors. We performed a cross-analysis of the cell-scaffold interactions (spreading, organization, and specific gene expression) with mechanical (elasticity), morphological (porosity, fibers diameter and orientation) and surface (wettability) characterizations of the electrospun fibers. We concluded that (1) osteogenic differentiation can be initiated on pure PCL-based electrospun scaffolds without specific culture conditions; (2) fiber alignment modified cell organization in the short term and (3) PLA added to PCL with an increased fiber diameter encouraged the stem cells towards the tendon lineage without additional tenogenic factors. In summary, the differentiation potential of stem cells on adapted electrospun fibers could be achieved in factor-free medium, making possible future applications in clinically relevant situations.

AB - The differentiation potential of mesenchymal stem cells (MSC) has been extensively tested on electrospun scaffolds. However, this potential is often assessed with lineage-specific medium, making it difficult to interpret the real contribution of the properties of the scaffold in the cell response. In this study, we analyzed the ability of different polycaprolactone/polylactic acid PCL/PLA electrospun scaffolds (pure or blended compositions, random or aligned fibers, various fiber diameters) to drive MSC towards bone or tendon lineages in the absence of specific differentiation medium. C3H10T1/2 cells (a mesenchymal stem cell model) were cultured on scaffolds for 96 h without differentiation factors. We performed a cross-analysis of the cell-scaffold interactions (spreading, organization, and specific gene expression) with mechanical (elasticity), morphological (porosity, fibers diameter and orientation) and surface (wettability) characterizations of the electrospun fibers. We concluded that (1) osteogenic differentiation can be initiated on pure PCL-based electrospun scaffolds without specific culture conditions; (2) fiber alignment modified cell organization in the short term and (3) PLA added to PCL with an increased fiber diameter encouraged the stem cells towards the tendon lineage without additional tenogenic factors. In summary, the differentiation potential of stem cells on adapted electrospun fibers could be achieved in factor-free medium, making possible future applications in clinically relevant situations.

KW - Cell differentiation

KW - Electrospinning

KW - Mesenchymal stem cell

KW - Polymer

KW - Scaffold

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85037329901&partnerID=8YFLogxK

U2 - 10.3390/ma10121387

DO - 10.3390/ma10121387

M3 - Article

VL - 10

JO - Materials

JF - Materials

SN - 1996-1944

IS - 12

M1 - 1387

ER -