The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Koji Kawamura
  • Yoshihiro Ueda
  • Shogo Matsumoto
  • Takanori Horibe
  • Shungo Otagaki
  • Li Wang
  • Guoliang Wang
  • Laurence Hibrand-Saint Oyant
  • Fabrice Foucher
  • Marcus Linde
  • Thomas Debener

Externe Organisationen

  • Osaka Institute of Technology
  • Gifu International Academy of Horticulture
  • Gifu World Rose Garden
  • Universität Nagoya
  • Chubu University
  • Sichuan University
  • Jiangsu Provincial Department of Agriculture and Rural Affairs
  • Nanjing Agricultural University
  • Université d'Angers
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummeruhac155
FachzeitschriftHorticulture research
Jahrgang9
PublikationsstatusVeröffentlicht - 1 Okt. 2022

Abstract

This study aims to: (i) identify the Rosa S-locus controlling self-incompatibility (SI); (ii) test the genetic linkage of the S-locus with other loci controlling important ornamental traits, such as the continuous-flowering (CF) characteristic; (iii) identify the S-alleles (SC) of old Chinese CF cultivars (e.g, Old Blush, Slater's Crimson China) and examine the changes in the frequency of cultivars with Sc through the history of breeding; (iv) identify wild species carrying the Sc-alleles to infer wild origins of CF cultivars. We identified a new S-RNase (SC2) of Rosa chinensis in a contig from a genome database that has not been integrated into one of the seven chromosomes yet. Genetic mapping indicated that SC2 is allelic to the previously-identified S-RNase (SC1) in chromosome 3. Pollination experiments with half-compatible pairs of roses confirmed that they are the pistil-determinant of SI. The segregation analysis of an F1-population indicated genetic linkage between the S-locus and the floral repressor gene KSN. The non-functional allele ksn is responsible for the CF characteristic. A total of five S-alleles (SC1-5) were identified from old CF cultivars. The frequency of cultivars with SC dramatically increased after the introgression of ksn from Chinese to European cultivars and remains high (80%) in modern cultivars, suggesting that S-genotyping is helpful for effective breeding. Wild individuals carrying SC were found in Rosa multiflora (SC1), Rosa chinensis var. spontanea (SC3), and Rosa gigantea (SC2, SC4), supporting the hypothesis of hybrid origins of CF cultivars and providing a new evidence for the involvement of Rosa multiflora.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biotechnologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biochemie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik
  • Agrar- und Biowissenschaften (insg.)
  • Pflanzenkunde
  • Agrar- und Biowissenschaften (insg.)
  • Gartenbau

Zitieren

The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses. / Kawamura, Koji; Ueda, Yoshihiro; Matsumoto, Shogo et al.
in: Horticulture research, Jahrgang 9, uhac155, 01.10.2022.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Kawamura, K, Ueda, Y, Matsumoto, S, Horibe, T, Otagaki, S, Wang, L, Wang, G, Hibrand-Saint Oyant, L, Foucher, F, Linde, M & Debener, T 2022, 'The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses', Horticulture research, Jg. 9, uhac155. https://doi.org/10.1093/hr/uhac155
Kawamura, K., Ueda, Y., Matsumoto, S., Horibe, T., Otagaki, S., Wang, L., Wang, G., Hibrand-Saint Oyant, L., Foucher, F., Linde, M., & Debener, T. (2022). The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses. Horticulture research, 9, Artikel uhac155. https://doi.org/10.1093/hr/uhac155
Kawamura K, Ueda Y, Matsumoto S, Horibe T, Otagaki S, Wang L et al. The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses. Horticulture research. 2022 Okt 1;9:uhac155. doi: 10.1093/hr/uhac155
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title = "The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses",
abstract = "This study aims to: (i) identify the Rosa S-locus controlling self-incompatibility (SI); (ii) test the genetic linkage of the S-locus with other loci controlling important ornamental traits, such as the continuous-flowering (CF) characteristic; (iii) identify the S-alleles (SC) of old Chinese CF cultivars (e.g, Old Blush, Slater's Crimson China) and examine the changes in the frequency of cultivars with Sc through the history of breeding; (iv) identify wild species carrying the Sc-alleles to infer wild origins of CF cultivars. We identified a new S-RNase (SC2) of Rosa chinensis in a contig from a genome database that has not been integrated into one of the seven chromosomes yet. Genetic mapping indicated that SC2 is allelic to the previously-identified S-RNase (SC1) in chromosome 3. Pollination experiments with half-compatible pairs of roses confirmed that they are the pistil-determinant of SI. The segregation analysis of an F1-population indicated genetic linkage between the S-locus and the floral repressor gene KSN. The non-functional allele ksn is responsible for the CF characteristic. A total of five S-alleles (SC1-5) were identified from old CF cultivars. The frequency of cultivars with SC dramatically increased after the introgression of ksn from Chinese to European cultivars and remains high (80%) in modern cultivars, suggesting that S-genotyping is helpful for effective breeding. Wild individuals carrying SC were found in Rosa multiflora (SC1), Rosa chinensis var. spontanea (SC3), and Rosa gigantea (SC2, SC4), supporting the hypothesis of hybrid origins of CF cultivars and providing a new evidence for the involvement of Rosa multiflora.",
author = "Koji Kawamura and Yoshihiro Ueda and Shogo Matsumoto and Takanori Horibe and Shungo Otagaki and Li Wang and Guoliang Wang and {Hibrand-Saint Oyant}, Laurence and Fabrice Foucher and Marcus Linde and Thomas Debener",
note = "Funding Information: Acknowledgements This study was supported by the JSPS KAKENHI No. 24688004 and 17H04616. We would like to express our appreciation for Cheng Tingting and Jin Tang in Sichuan University, and Ryosuke Sakamoto in Osaka Institute of Technology, for their helpful support during the fieldwork in China. We are also appreciative of the support from The Rose Culture Institute, Japan, and Drs. Masaki Ochiai and Kunio Yamada at Gifu University, for providing rose materials.",
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Download

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T1 - The identification of the Rosa S-locus provides new insights into the breeding and wild origins of continuous-flowering roses

AU - Kawamura, Koji

AU - Ueda, Yoshihiro

AU - Matsumoto, Shogo

AU - Horibe, Takanori

AU - Otagaki, Shungo

AU - Wang, Li

AU - Wang, Guoliang

AU - Hibrand-Saint Oyant, Laurence

AU - Foucher, Fabrice

AU - Linde, Marcus

AU - Debener, Thomas

N1 - Funding Information: Acknowledgements This study was supported by the JSPS KAKENHI No. 24688004 and 17H04616. We would like to express our appreciation for Cheng Tingting and Jin Tang in Sichuan University, and Ryosuke Sakamoto in Osaka Institute of Technology, for their helpful support during the fieldwork in China. We are also appreciative of the support from The Rose Culture Institute, Japan, and Drs. Masaki Ochiai and Kunio Yamada at Gifu University, for providing rose materials.

PY - 2022/10/1

Y1 - 2022/10/1

N2 - This study aims to: (i) identify the Rosa S-locus controlling self-incompatibility (SI); (ii) test the genetic linkage of the S-locus with other loci controlling important ornamental traits, such as the continuous-flowering (CF) characteristic; (iii) identify the S-alleles (SC) of old Chinese CF cultivars (e.g, Old Blush, Slater's Crimson China) and examine the changes in the frequency of cultivars with Sc through the history of breeding; (iv) identify wild species carrying the Sc-alleles to infer wild origins of CF cultivars. We identified a new S-RNase (SC2) of Rosa chinensis in a contig from a genome database that has not been integrated into one of the seven chromosomes yet. Genetic mapping indicated that SC2 is allelic to the previously-identified S-RNase (SC1) in chromosome 3. Pollination experiments with half-compatible pairs of roses confirmed that they are the pistil-determinant of SI. The segregation analysis of an F1-population indicated genetic linkage between the S-locus and the floral repressor gene KSN. The non-functional allele ksn is responsible for the CF characteristic. A total of five S-alleles (SC1-5) were identified from old CF cultivars. The frequency of cultivars with SC dramatically increased after the introgression of ksn from Chinese to European cultivars and remains high (80%) in modern cultivars, suggesting that S-genotyping is helpful for effective breeding. Wild individuals carrying SC were found in Rosa multiflora (SC1), Rosa chinensis var. spontanea (SC3), and Rosa gigantea (SC2, SC4), supporting the hypothesis of hybrid origins of CF cultivars and providing a new evidence for the involvement of Rosa multiflora.

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