TY - JOUR

T1 - The gene space of European mistletoe (Viscum album)

AU - Schröder, Lucie

AU - Hohnjec, Natalija

AU - Senkler, Michael

AU - Senkler, Jennifer

AU - Küster, Helge

AU - Braun, Hans-Peter

N1 - Funding Information: We thank Dr. Sabine Reichwein for project advice, Marius Schrader for taking care of the apple tree used for cultivation and Matthias Döring for critical reading of the manuscript. PacBio SMRT sequencing was carried out by Novogene, Cambridge, UK. This research has been supported by the Deutsche Forschungsgemeinschaft (grant BR 1829/16‐1 to HPB). Open access funding enabled and organized by Projekt DEAL. V. album

PY - 2021/12

Y1 - 2021/12

N2 - European mistletoe is a hemiparasitic flowering plant that is known for a very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5' and 3' non-coding regions). Messenger-RNA fractions were isolated from different V. album organs harvested in summer or winter and were analysed via Single Molecule Real-Time sequencing. We determined sequences of 39,092 distinct open reading frames encoding 32,064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry based data of the V. album mitochondrial proteome, which previously had been evaluated using the Arabidopsis thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.

AB - European mistletoe is a hemiparasitic flowering plant that is known for a very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5' and 3' non-coding regions). Messenger-RNA fractions were isolated from different V. album organs harvested in summer or winter and were analysed via Single Molecule Real-Time sequencing. We determined sequences of 39,092 distinct open reading frames encoding 32,064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry based data of the V. album mitochondrial proteome, which previously had been evaluated using the Arabidopsis thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.

KW - Arabidopsis thaliana

KW - SMRT sequencing

KW - Viscum album

KW - complex I

KW - lectins

KW - mitochondria

KW - oxidative phosphorylation

KW - viscotoxins

UR - http://www.scopus.com/inward/record.url?scp=85119138581&partnerID=8YFLogxK

U2 - 10.1111/tpj.15558

DO - 10.1111/tpj.15558

M3 - Article

C2 - 34713513

VL - 109

SP - 278

EP - 294

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 1

ER -