TY - JOUR

T1 - The gene diversity pattern of Diplocarpon rosae populations is shaped by the age, diversity and fungicide treatment of their host populations

AU - Münnekhoff, A. K.

AU - Linde, M.

AU - Debener, T.

N1 - Funding information: The authors would like to thank the three rose breeding companies NOACK ROSEN Baum- und Rosenschulen, Rosen Tantau Vertrieb GmbH & Co. KG and W. Kordes’ Söhne Rosenschulen GmbH & Co. KG for their support. The work was funded by the German Federation of Industrial Research Associations (AiF) in the programme for applied research and development (R&D) for small and medium-sized enterprises (SMEs). The authors have no conflicts of interest to declare.

PY - 2017/9/14

Y1 - 2017/9/14

N2 - Black spot disease caused by the hemibiotrophic ascomycete Diplocarpon rosae is the most devastating disease of field-grown roses. Although resistance to black spot is an important trait for rose breeding, little information on the diversity of the pathogen is currently available. To date, a number of single-spore isolates have been characterized based on a set of test genotypes of the host. In this study, six polymorphic simple sequence repeat (SSR) markers for D. rosae were developed and their potential application to single-spore isolates collected from a wide range of geographic locations within and outside Europe discussed. Populations of the fungus were then analysed in different German rose collections/populations. Gene diversity was highest in older rose collections managed without fungicide application, and lowest in the two- to three-year-old testing sites of German rose breeders. Additional analyses of a global collection of single samples and populations revealed no unique alleles from any of the locations, indicating that global trading of rose varieties has led to an admixture of the pathogen.

AB - Black spot disease caused by the hemibiotrophic ascomycete Diplocarpon rosae is the most devastating disease of field-grown roses. Although resistance to black spot is an important trait for rose breeding, little information on the diversity of the pathogen is currently available. To date, a number of single-spore isolates have been characterized based on a set of test genotypes of the host. In this study, six polymorphic simple sequence repeat (SSR) markers for D. rosae were developed and their potential application to single-spore isolates collected from a wide range of geographic locations within and outside Europe discussed. Populations of the fungus were then analysed in different German rose collections/populations. Gene diversity was highest in older rose collections managed without fungicide application, and lowest in the two- to three-year-old testing sites of German rose breeders. Additional analyses of a global collection of single samples and populations revealed no unique alleles from any of the locations, indicating that global trading of rose varieties has led to an admixture of the pathogen.

KW - black spot

KW - gene diversity

KW - microsatellite markers

KW - roses

UR - http://www.scopus.com/inward/record.url?scp=85014439331&partnerID=8YFLogxK

U2 - 10.1111/ppa.12681

DO - 10.1111/ppa.12681

M3 - Article

AN - SCOPUS:85014439331

VL - 66

SP - 1288

EP - 1298

JO - Plant pathology

JF - Plant pathology

SN - 0032-0862

IS - 8

ER -