TY - JOUR

T1 - The establishment of effective regeneration system and in vitro culture of Campanula

AU - Karimiani, Z. Ghayoor

AU - Gehl, Christian

AU - Serek, Margrethe

PY - 2020/7/8

Y1 - 2020/7/8

N2 - Several species of Campanula genus have been cultivated as ornamental plants, and there is an increasing demand for more attractive and novel characteristics. Optimal regeneration protocol is prerequisite for subsequent successful genetic manipulation and generation of new features. In the current study, the optimal media composition and explant source for shoot regeneration of three Campanula cultivars, C. portenschlagiana 'Blue Ocean' (BO) and 'Royal', C. carpatica 'Improved Blue Uniform' (IBU) were determined. The focus of this study was based on avoiding an over-supplementation of plant growth regulators (PGRs), especially the cytokinin thidiazuron (TDZ). The explants examined for shoot regeneration were petioles harvested from two different explant sources: 1) petioles of 3-month-old tissue-cultured plants, 2) petioles of etiolated nodal cuts. To find out the best media composition for shoot regeneration, different naphthalene acetic acid (0.5, 1.0 and 2.0 mg L-1) and TDZ (1.0, 2.0, 3.0 and 5.0 mg L-1) concentrations were investigated. According to the results, the most favourable explant source for harvesting petioles were etiolated nodal cuts of mature greenhouse plants. The best shoot regeneration obtained from the harvested petioles of nodal cuts in the 3 cultivars were 85% in IBU, 65% in BO and 45.8% in 'Royal'.

AB - Several species of Campanula genus have been cultivated as ornamental plants, and there is an increasing demand for more attractive and novel characteristics. Optimal regeneration protocol is prerequisite for subsequent successful genetic manipulation and generation of new features. In the current study, the optimal media composition and explant source for shoot regeneration of three Campanula cultivars, C. portenschlagiana 'Blue Ocean' (BO) and 'Royal', C. carpatica 'Improved Blue Uniform' (IBU) were determined. The focus of this study was based on avoiding an over-supplementation of plant growth regulators (PGRs), especially the cytokinin thidiazuron (TDZ). The explants examined for shoot regeneration were petioles harvested from two different explant sources: 1) petioles of 3-month-old tissue-cultured plants, 2) petioles of etiolated nodal cuts. To find out the best media composition for shoot regeneration, different naphthalene acetic acid (0.5, 1.0 and 2.0 mg L-1) and TDZ (1.0, 2.0, 3.0 and 5.0 mg L-1) concentrations were investigated. According to the results, the most favourable explant source for harvesting petioles were etiolated nodal cuts of mature greenhouse plants. The best shoot regeneration obtained from the harvested petioles of nodal cuts in the 3 cultivars were 85% in IBU, 65% in BO and 45.8% in 'Royal'.

KW - Explant source

KW - PGRs

KW - Shoot regeneration

KW - TDZ concentration

KW - Thidiazuron

UR - http://www.scopus.com/inward/record.url?scp=85090688349&partnerID=8YFLogxK

U2 - 10.17660/ActaHortic.2020.1285.2

DO - 10.17660/ActaHortic.2020.1285.2

M3 - Article

AN - SCOPUS:85090688349

VL - 1285

SP - 9

EP - 14

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -