Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 39-45 |
| Seitenumfang | 7 |
| Fachzeitschrift | Plant Cell, Tissue and Organ Culture |
| Jahrgang | 59 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 1999 |
| Extern publiziert | Ja |
Abstract
The effect of the relative oxygen partial pressure (pO2) in bioreactors on cell proliferation and subsequent differentiation of somatic embryos from suspension cultures of Cyclamen persicum Mill. was investigated. The growth rate of cell line 3738-VIII in growth-regulator containing medium in bioreactors at 5% pO2 was slightly reduced in comparison to 10% and 20% pO2. Cultures growing at 40% pO2 had a lower growth rate, a markedly reduced cell viability and showed a decrease of the medium pH to 3.5. Because a pH-control with a setpoint of 3.3 caused cell death within 4 days, it was assumed, that the reason for the poor cell proliferation and viability in the cultures at 40% pO2 was an effect of medium acidification rather than of the high O2 partial pressure. A significantly higher number of germinating embryos was obtained from the cultures grown at 40% pO2 than from those grown in flasks or in bioreactors at 5%, 10% and 20% pO2. These results were specific for cell line 3738-VIII. Another cell line, 3736-12, did not show marked differences in cell proliferation, viability, pH or subsequent regeneration of somatic embryos when grown at different O2 partial pressures.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Gartenbau
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in: Plant Cell, Tissue and Organ Culture, Jahrgang 59, Nr. 1, 1999, S. 39-45.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The effect of oxygen partial pressure in bioreactors on cell proliferation and subsequent differentiation of somatic embryos of Cyclamen persicum
AU - Hohe, Annette
AU - Winkelmann, Traud
AU - Schwenkel, Hans Georg
PY - 1999
Y1 - 1999
N2 - The effect of the relative oxygen partial pressure (pO2) in bioreactors on cell proliferation and subsequent differentiation of somatic embryos from suspension cultures of Cyclamen persicum Mill. was investigated. The growth rate of cell line 3738-VIII in growth-regulator containing medium in bioreactors at 5% pO2 was slightly reduced in comparison to 10% and 20% pO2. Cultures growing at 40% pO2 had a lower growth rate, a markedly reduced cell viability and showed a decrease of the medium pH to 3.5. Because a pH-control with a setpoint of 3.3 caused cell death within 4 days, it was assumed, that the reason for the poor cell proliferation and viability in the cultures at 40% pO2 was an effect of medium acidification rather than of the high O2 partial pressure. A significantly higher number of germinating embryos was obtained from the cultures grown at 40% pO2 than from those grown in flasks or in bioreactors at 5%, 10% and 20% pO2. These results were specific for cell line 3738-VIII. Another cell line, 3736-12, did not show marked differences in cell proliferation, viability, pH or subsequent regeneration of somatic embryos when grown at different O2 partial pressures.
AB - The effect of the relative oxygen partial pressure (pO2) in bioreactors on cell proliferation and subsequent differentiation of somatic embryos from suspension cultures of Cyclamen persicum Mill. was investigated. The growth rate of cell line 3738-VIII in growth-regulator containing medium in bioreactors at 5% pO2 was slightly reduced in comparison to 10% and 20% pO2. Cultures growing at 40% pO2 had a lower growth rate, a markedly reduced cell viability and showed a decrease of the medium pH to 3.5. Because a pH-control with a setpoint of 3.3 caused cell death within 4 days, it was assumed, that the reason for the poor cell proliferation and viability in the cultures at 40% pO2 was an effect of medium acidification rather than of the high O2 partial pressure. A significantly higher number of germinating embryos was obtained from the cultures grown at 40% pO2 than from those grown in flasks or in bioreactors at 5%, 10% and 20% pO2. These results were specific for cell line 3738-VIII. Another cell line, 3736-12, did not show marked differences in cell proliferation, viability, pH or subsequent regeneration of somatic embryos when grown at different O2 partial pressures.
KW - Cell viability
KW - Medium acidification
KW - pH
KW - pO
KW - Suspension culture
UR - http://www.scopus.com/inward/record.url?scp=0033506967&partnerID=8YFLogxK
U2 - 10.1023/A:1006323009860
DO - 10.1023/A:1006323009860
M3 - Article
AN - SCOPUS:0033506967
VL - 59
SP - 39
EP - 45
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -