Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 343-352 |
| Seitenumfang | 10 |
| Fachzeitschrift | Journal of Drug Delivery Science and Technology |
| Jahrgang | 35 |
| Frühes Online-Datum | 2 Sept. 2016 |
| Publikationsstatus | Veröffentlicht - Okt. 2016 |
Abstract
An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whether we could generate a DDS with PCL by electrospinning. By characterizing the fibers’ structural properties we found that fibers’ diameters were affected by the concentration of DIP, which consequently had an impact on the surface area-to-volume ratio and therefore to cumulative drug release. In vitro cell studies on a bovine aortic endothelial cell line showed that PCL itself did not affect the cells according to analysis of the number of viable cells over time and metabolic activity. We found that DIP which was released from the electrospun fibers in the solution was accumulated in the cells and that it had similar effects on the cells as it had directly delivered to them. DIP inhibited the cell growth and enhanced the gap junction coupling of the endothelial cells. The results indicate that the encapsulation and the electrospinning did not affect the biological activity of DIP and show that the method can be used to generate a DDS.
ASJC Scopus Sachgebiete
- Pharmakologie, Toxikologie und Pharmazie (insg.)
- Pharmazeutische Wissenschaften
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in: Journal of Drug Delivery Science and Technology, Jahrgang 35, 10.2016, S. 343-352.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The effect of dipyridamole embedded in a drug delivery system made by electrospun nanofibers on aortic endothelial cells
AU - Repanas, A.
AU - Bader, A.
AU - Klett, A.
AU - Ngezahayo, A.
AU - Glasmacher, B.
N1 - Funding Information: This research was granted (DFG EXC 62/1) by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) and by the Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK) in the joint project SynFoBiA – “Novel synthesis and formulation methods for poorly soluble drugs and sensitive biopharmaceuticals”.
PY - 2016/10
Y1 - 2016/10
N2 - An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whether we could generate a DDS with PCL by electrospinning. By characterizing the fibers’ structural properties we found that fibers’ diameters were affected by the concentration of DIP, which consequently had an impact on the surface area-to-volume ratio and therefore to cumulative drug release. In vitro cell studies on a bovine aortic endothelial cell line showed that PCL itself did not affect the cells according to analysis of the number of viable cells over time and metabolic activity. We found that DIP which was released from the electrospun fibers in the solution was accumulated in the cells and that it had similar effects on the cells as it had directly delivered to them. DIP inhibited the cell growth and enhanced the gap junction coupling of the endothelial cells. The results indicate that the encapsulation and the electrospinning did not affect the biological activity of DIP and show that the method can be used to generate a DDS.
AB - An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whether we could generate a DDS with PCL by electrospinning. By characterizing the fibers’ structural properties we found that fibers’ diameters were affected by the concentration of DIP, which consequently had an impact on the surface area-to-volume ratio and therefore to cumulative drug release. In vitro cell studies on a bovine aortic endothelial cell line showed that PCL itself did not affect the cells according to analysis of the number of viable cells over time and metabolic activity. We found that DIP which was released from the electrospun fibers in the solution was accumulated in the cells and that it had similar effects on the cells as it had directly delivered to them. DIP inhibited the cell growth and enhanced the gap junction coupling of the endothelial cells. The results indicate that the encapsulation and the electrospinning did not affect the biological activity of DIP and show that the method can be used to generate a DDS.
KW - DDS
KW - Dipyridamole
KW - Electrospinning
KW - Endothelial cells
KW - Gap junction coupling
KW - Polycaprolactone
UR - http://www.scopus.com/inward/record.url?scp=84986537122&partnerID=8YFLogxK
U2 - 10.1016/j.jddst.2016.08.011
DO - 10.1016/j.jddst.2016.08.011
M3 - Article
VL - 35
SP - 343
EP - 352
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
SN - 1157-1489
ER -