TY - JOUR

T1 - The contribution of ammonia-oxidizing archaea and bacteria to gross nitrification under different substrate availability

AU - Rütting, Tobias

AU - Schleusner, Philipp

AU - Hink, Linda

AU - Prosser, James I.

N1 - Funding Information: The work was supported by the European Union ( Marie Curie ITN NORA , FP7- 316472 ). This publication is part of the Swedish strategic research area ‘‘Biodiversity and Ecosystem services in a Changing Climate - BECC’’ ( www.becc.lu.se/ ).

PY - 2021/9

Y1 - 2021/9

N2 - The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined by the substrate sources and availability, yet evidence provided by quantification of their respective gross activities in soil is lacking. Here, we conducted a microcosm study with agricultural soil with high (50 μg N g −1) and low (5 μg N g −1) ammonium application, and quantified gross nitrification rates using 15N-tracers. AOA and AOB activities were distinguished using the bacterial inhibitor 1-octyne and acetylene, which inhibits both AOA and AOB. Under low ammonium supply, AOA and AOB contributed equally to gross ammonia oxidation, but AOB outcompeted AOA under higher ammonium supply. These results provide the first direct evidence that substrate availability affects the relative contribution of AOA and AOB to gross nitrification.

AB - The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined by the substrate sources and availability, yet evidence provided by quantification of their respective gross activities in soil is lacking. Here, we conducted a microcosm study with agricultural soil with high (50 μg N g −1) and low (5 μg N g −1) ammonium application, and quantified gross nitrification rates using 15N-tracers. AOA and AOB activities were distinguished using the bacterial inhibitor 1-octyne and acetylene, which inhibits both AOA and AOB. Under low ammonium supply, AOA and AOB contributed equally to gross ammonia oxidation, but AOB outcompeted AOA under higher ammonium supply. These results provide the first direct evidence that substrate availability affects the relative contribution of AOA and AOB to gross nitrification.

KW - 1-Octyne

KW - Acetylene

KW - Ammonia oxidation

KW - N-tracing

UR - http://www.scopus.com/inward/record.url?scp=85109210657&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2021.108353

DO - 10.1016/j.soilbio.2021.108353

M3 - Article

VL - 160

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 108353

ER -