Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 205-209 |
| Seitenumfang | 5 |
| Fachzeitschrift | FEBS letters |
| Jahrgang | 431 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 17 Juli 1998 |
| Extern publiziert | Ja |
Abstract
In Chlamydomonas reinhardtii, molybdopterin cofactar (MoCo) able to reconstitute active nitrate reductase (NR) with apoenzyme from the Neurospora crassa mutant nit-1 was found mostly bound to a carrier protein (CP). This protein is scarce in the algal free extracts and has been purified 520-fold. MoCoCP is a protein of 64 kDa with subunits of 16.5 kDa and an isoelectric point of 4.5. In contrast to free MoCo, MoCo bound to CP was remarkably protected against inactivation under both aerobic conditions and basic pH. MocoCP transferred active MoCo to apoNR in vitro without addition of molybdate, though reconstituted activity was 20% higher in the presence of molybdate. Incubation with tungstate specifically inhibited MoCoCP activity but had no effect an the activity of free MoCo released from milk xanthine oxidase. MoCoCP did not charge molybdate unless in the presence of N. crassa extracts. Our data support that MoCoCP stabilizes MoCo in an active form charged with molybdate to provide MoCo to apomolybdoenzymes.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Strukturelle Biologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Zellbiologie
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in: FEBS letters, Jahrgang 431, Nr. 2, 17.07.1998, S. 205-209.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The Chlamydomonas reinhardtii MoCo carrier protein is multimeric and stabilizes molybdopteria cofactor in a molybdate charged form
AU - Witte, Claus Peter
AU - Igeño, M. Isabel
AU - Mendel, Ralf
AU - Schwarz, Günter
AU - Fernández, Emilio
PY - 1998/7/17
Y1 - 1998/7/17
N2 - In Chlamydomonas reinhardtii, molybdopterin cofactar (MoCo) able to reconstitute active nitrate reductase (NR) with apoenzyme from the Neurospora crassa mutant nit-1 was found mostly bound to a carrier protein (CP). This protein is scarce in the algal free extracts and has been purified 520-fold. MoCoCP is a protein of 64 kDa with subunits of 16.5 kDa and an isoelectric point of 4.5. In contrast to free MoCo, MoCo bound to CP was remarkably protected against inactivation under both aerobic conditions and basic pH. MocoCP transferred active MoCo to apoNR in vitro without addition of molybdate, though reconstituted activity was 20% higher in the presence of molybdate. Incubation with tungstate specifically inhibited MoCoCP activity but had no effect an the activity of free MoCo released from milk xanthine oxidase. MoCoCP did not charge molybdate unless in the presence of N. crassa extracts. Our data support that MoCoCP stabilizes MoCo in an active form charged with molybdate to provide MoCo to apomolybdoenzymes.
AB - In Chlamydomonas reinhardtii, molybdopterin cofactar (MoCo) able to reconstitute active nitrate reductase (NR) with apoenzyme from the Neurospora crassa mutant nit-1 was found mostly bound to a carrier protein (CP). This protein is scarce in the algal free extracts and has been purified 520-fold. MoCoCP is a protein of 64 kDa with subunits of 16.5 kDa and an isoelectric point of 4.5. In contrast to free MoCo, MoCo bound to CP was remarkably protected against inactivation under both aerobic conditions and basic pH. MocoCP transferred active MoCo to apoNR in vitro without addition of molybdate, though reconstituted activity was 20% higher in the presence of molybdate. Incubation with tungstate specifically inhibited MoCoCP activity but had no effect an the activity of free MoCo released from milk xanthine oxidase. MoCoCP did not charge molybdate unless in the presence of N. crassa extracts. Our data support that MoCoCP stabilizes MoCo in an active form charged with molybdate to provide MoCo to apomolybdoenzymes.
KW - Chlamydomonas reinhardtii
KW - Molybdenum cofactor
KW - Molybdopterin
KW - Tungstate
UR - http://www.scopus.com/inward/record.url?scp=0032540896&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00756-X
DO - 10.1016/S0014-5793(98)00756-X
M3 - Article
C2 - 9708903
AN - SCOPUS:0032540896
VL - 431
SP - 205
EP - 209
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 2
ER -