Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 4926-4929 |
| Seitenumfang | 4 |
| Fachzeitschrift | Angewandte Chemie |
| Jahrgang | 46 |
| Ausgabenummer | 26 |
| Publikationsstatus | Veröffentlicht - 18 Juli 2007 |
| Extern publiziert | Ja |
Abstract
(Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
ASJC Scopus Sachgebiete
- Chemische Verfahrenstechnik (insg.)
- Katalyse
- Chemie (insg.)
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in: Angewandte Chemie , Jahrgang 46, Nr. 26, 18.07.2007, S. 4926-4929.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coli
AU - Wong, Ursula
AU - Cox, Russell J.
PY - 2007/7/18
Y1 - 2007/7/18
N2 - (Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
AB - (Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
KW - Enzymes
KW - Isotopic labeling
KW - Kinetic isotope effects
KW - Reaction mechanisms
UR - http://www.scopus.com/inward/record.url?scp=34447344507&partnerID=8YFLogxK
U2 - 10.1002/anie.200700647
DO - 10.1002/anie.200700647
M3 - Article
C2 - 17516600
AN - SCOPUS:34447344507
VL - 46
SP - 4926
EP - 4929
JO - Angewandte Chemie
JF - Angewandte Chemie
SN - 1433-7851
IS - 26
ER -