TY - JOUR

T1 - The barley HvSTP13GR mutant triggers resistance against biotrophic fungi

AU - Skoppek, Caroline Ines

AU - Punt, Wilko

AU - Heinrichs, Marleen

AU - Ordon, Frank

AU - Wehner, Gwendolin

AU - Boch, Jens

AU - Streubel, Jana

N1 - Funding Information: We thank Anna Marthe, Christine Hoppe, and Ilona Renneberg for technical assistance and Sebastian Becker and Annekatrin Richter for helpful discussions on the manuscript. We are grateful to Claude Bragard and Ralf Koebnik for providing Xth UPB820 and Alain Tissier for providing yeast MoClo plasmids. This work was supported by university core funding only. R

PY - 2022/1/9

Y1 - 2022/1/9

N2 - High-yielding and stress-resistant crops are essential to ensure future food supply. Barley is an important crop to feed livestock and to produce malt, but the annual yield is threatened by pathogen infections. Pathogens can trigger an altered sugar partitioning in the host plant, which possibly leads to an advantage for the pathogen. Hampering these processes represents a promising strategy to potentially increase resistance. We analysed the response of the barley monosaccharide transporter HvSTP13 towards biotic stress and its potential use for plant protection. The expression of HvSTP13 increased on bacterial and fungal pathogen-associated molecular pattern (PAMP) application, suggesting a PAMP-triggered signalling that converged on the transcriptional induction of the gene. Promoter studies indicate a region that is probably targeted by transcription factors downstream of PAMP-triggered immunity pathways. We confirmed that the nonfunctional HvSTP13GR variant confers resistance against an economically relevant biotrophic rust fungus in barley. Our experimental setup provides basal prerequisites to further decode the role of HvSTP13 in response to biological stress. Moreover, in line with other studies, our experiments indicate that the alteration of sugar partitioning pathways, in a host–pathogen interaction, is a promising approach to achieve broad and durable resistance in plants.

AB - High-yielding and stress-resistant crops are essential to ensure future food supply. Barley is an important crop to feed livestock and to produce malt, but the annual yield is threatened by pathogen infections. Pathogens can trigger an altered sugar partitioning in the host plant, which possibly leads to an advantage for the pathogen. Hampering these processes represents a promising strategy to potentially increase resistance. We analysed the response of the barley monosaccharide transporter HvSTP13 towards biotic stress and its potential use for plant protection. The expression of HvSTP13 increased on bacterial and fungal pathogen-associated molecular pattern (PAMP) application, suggesting a PAMP-triggered signalling that converged on the transcriptional induction of the gene. Promoter studies indicate a region that is probably targeted by transcription factors downstream of PAMP-triggered immunity pathways. We confirmed that the nonfunctional HvSTP13GR variant confers resistance against an economically relevant biotrophic rust fungus in barley. Our experimental setup provides basal prerequisites to further decode the role of HvSTP13 in response to biological stress. Moreover, in line with other studies, our experiments indicate that the alteration of sugar partitioning pathways, in a host–pathogen interaction, is a promising approach to achieve broad and durable resistance in plants.

KW - HvSTP13

KW - PAMP

KW - barley

KW - fungi

KW - resistance

KW - sugar

UR - http://www.scopus.com/inward/record.url?scp=85119657824&partnerID=8YFLogxK

U2 - 10.1111/mpp.13161

DO - 10.1111/mpp.13161

M3 - Article

VL - 23

SP - 278

EP - 290

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

SN - 1464-6722

IS - 2

ER -