Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 55-62 |
| Seitenumfang | 8 |
| Fachzeitschrift | Analytica chimica acta |
| Jahrgang | 313 |
| Ausgabenummer | 1-2 |
| Publikationsstatus | Veröffentlicht - 20 Sept. 1995 |
| Extern publiziert | Ja |
Abstract
In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its application for two different flow-injection immunoassays. Both systems are based on the principles of flow-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell cultivation processes. One system uses a homogeneous assay with turbidimetric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line application during a mammalian cell cultivation as well as off-line measurements of medium samples are presented. The results obtained from on-line measurements are compared to ELISA results. The automation of the analysis systems with respect to its flexible control as well as the reliable evaluation of the measurement signals -even if they are severely distorted -are discussed in detail.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Umweltwissenschaften (insg.)
- Umweltchemie
- Chemie (insg.)
- Spektroskopie
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in: Analytica chimica acta, Jahrgang 313, Nr. 1-2, 20.09.1995, S. 55-62.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The automation of immun-FIA-systems
AU - Hitzmann, B.
AU - Löhn, A.
AU - Reinecke, M.
AU - Schulze, B.
AU - Scheper, T.
PY - 1995/9/20
Y1 - 1995/9/20
N2 - In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its application for two different flow-injection immunoassays. Both systems are based on the principles of flow-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell cultivation processes. One system uses a homogeneous assay with turbidimetric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line application during a mammalian cell cultivation as well as off-line measurements of medium samples are presented. The results obtained from on-line measurements are compared to ELISA results. The automation of the analysis systems with respect to its flexible control as well as the reliable evaluation of the measurement signals -even if they are severely distorted -are discussed in detail.
AB - In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its application for two different flow-injection immunoassays. Both systems are based on the principles of flow-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell cultivation processes. One system uses a homogeneous assay with turbidimetric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line application during a mammalian cell cultivation as well as off-line measurements of medium samples are presented. The results obtained from on-line measurements are compared to ELISA results. The automation of the analysis systems with respect to its flexible control as well as the reliable evaluation of the measurement signals -even if they are severely distorted -are discussed in detail.
KW - Automation
KW - Bioprocess monitoring
KW - Flow injection
KW - Immunoassay
KW - Reliable evaluation
UR - http://www.scopus.com/inward/record.url?scp=0029164558&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(95)00173-W
DO - 10.1016/0003-2670(95)00173-W
M3 - Article
AN - SCOPUS:0029164558
VL - 313
SP - 55
EP - 62
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 1-2
ER -