Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 89-95 |
| Seitenumfang | 7 |
| Fachzeitschrift | Plant science |
| Jahrgang | 124 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 18 Apr. 1997 |
| Extern publiziert | Ja |
Abstract
A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Plant science, Jahrgang 124, Nr. 1, 18.04.1997, S. 89-95.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules
AU - Küster, Helge
AU - Albus, Ulrike
AU - Frühling, Martin
AU - Tchetkova, Svetlana A.
AU - Tikhonovitch, Igor A.
AU - Pühler, Alfred
AU - Perlick, Andreas M.
N1 - Funding information: S.A. Tchetkova and I.A. Tikhonovitch acknowledge grants from the ‘Bundesministerium fur Ernahrung, Landwirtschaft und Forsten’ (BML).
PY - 1997/4/18
Y1 - 1997/4/18
N2 - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
AB - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
KW - Nodule nitrogen metabolism
KW - Nodule-specific cDNA library
KW - Tissue-print hybridization
UR - http://www.scopus.com/inward/record.url?scp=0343416950&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(97)04607-4
DO - 10.1016/S0168-9452(97)04607-4
M3 - Article
AN - SCOPUS:0343416950
VL - 124
SP - 89
EP - 95
JO - Plant science
JF - Plant science
SN - 0168-9452
IS - 1
ER -