Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1375-1384 |
| Seitenumfang | 10 |
| Fachzeitschrift | Journal of bacteriology |
| Jahrgang | 179 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 1 Jan. 1997 |
| Extern publiziert | Ja |
Abstract
Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661- 672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the biosynthesis of dTDP-glucose and dTDP-rhamnose, six glycosyltransferases, an ABC transporter complex homologous to the subfamily of peptide and protein export complexes, and a protein homologous to Rhizobium NodO proteins. In addition, homologies of three Exp proteins to transcriptional regulators, methyltransferases, and periplasmic binding proteins were found. The positions of 26 Tn5 insertions in the exp gene cluster were determined, thus allowing the previously described genetic map to be correlated with the sequence. Operon analysis revealed that the exp gene cluster consists of five complementation groups. In comparison to the wild-type background, all exp complementation groups were transcribed at a substantially elevated level in the regulatory mucR mutant.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
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in: Journal of bacteriology, Jahrgang 179, Nr. 4, 01.01.1997, S. 1375-1384.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan
T2 - Genetic organization and properties of the encoded gene products
AU - Becker, Anke
AU - Rüberg, Silvia
AU - Küster, Helge
AU - Roxlau, Andreas A.
AU - Keller, Mathias
AU - Ivashina, Tanja
AU - Cheng, Hai Ping
AU - Walker, Graham C.
AU - Pühler, Alfred
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661- 672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the biosynthesis of dTDP-glucose and dTDP-rhamnose, six glycosyltransferases, an ABC transporter complex homologous to the subfamily of peptide and protein export complexes, and a protein homologous to Rhizobium NodO proteins. In addition, homologies of three Exp proteins to transcriptional regulators, methyltransferases, and periplasmic binding proteins were found. The positions of 26 Tn5 insertions in the exp gene cluster were determined, thus allowing the previously described genetic map to be correlated with the sequence. Operon analysis revealed that the exp gene cluster consists of five complementation groups. In comparison to the wild-type background, all exp complementation groups were transcribed at a substantially elevated level in the regulatory mucR mutant.
AB - Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661- 672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the biosynthesis of dTDP-glucose and dTDP-rhamnose, six glycosyltransferases, an ABC transporter complex homologous to the subfamily of peptide and protein export complexes, and a protein homologous to Rhizobium NodO proteins. In addition, homologies of three Exp proteins to transcriptional regulators, methyltransferases, and periplasmic binding proteins were found. The positions of 26 Tn5 insertions in the exp gene cluster were determined, thus allowing the previously described genetic map to be correlated with the sequence. Operon analysis revealed that the exp gene cluster consists of five complementation groups. In comparison to the wild-type background, all exp complementation groups were transcribed at a substantially elevated level in the regulatory mucR mutant.
UR - http://www.scopus.com/inward/record.url?scp=0031058124&partnerID=8YFLogxK
U2 - 10.1128/jb.179.4.1375-1384.1997
DO - 10.1128/jb.179.4.1375-1384.1997
M3 - Article
C2 - 9023225
AN - SCOPUS:0031058124
VL - 179
SP - 1375
EP - 1384
JO - Journal of bacteriology
JF - Journal of bacteriology
SN - 0021-9193
IS - 4
ER -