Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Tatiana V. Kochetkova
  • Kseniya S. Zayulina
  • Vyacheslav S. Zhigarkov
  • Nikita V. Minaev
  • Boris N. Chichkov
  • Andrei A. Novikov
  • Stepan V. Toshchakov
  • Alexander G. Elcheninov
  • Ilya V. Kublanov

Externe Organisationen

  • I. M. Gubkin Russian State University of Oil and Gas
  • Russian Academy of Sciences (RAS)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer003902
Seiten (von - bis)1192-1202
Seitenumfang11
FachzeitschriftInternational Journal of Systematic and Evolutionary Microbiology
Jahrgang70
Ausgabenummer2
PublikationsstatusVeröffentlicht - Feb. 2020
Extern publiziertJa

Abstract

A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).

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Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14. / Kochetkova, Tatiana V.; Zayulina, Kseniya S.; Zhigarkov, Vyacheslav S. et al.
in: International Journal of Systematic and Evolutionary Microbiology, Jahrgang 70, Nr. 2, 003902, 02.2020, S. 1192-1202.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

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@article{35c99704809040bc8ff9e56224c6c6b9,
title = "Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14",
abstract = "A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).",
keywords = "Chloroflexi, Chukotka hot spring, Laser bioprinting, LEMS, Microbial dark matter, Tepidiforma, RNA, Ribosomal, 16S/genetics, Phospholipids/chemistry, Phylogeny, Sequence Analysis, DNA, Russia, Chloroflexi/classification, Hot Springs/microbiology, Carotenoids/chemistry, Arctic Regions, Base Composition, Bacterial Typing Techniques, Fatty Acids/chemistry, DNA, Bacterial/genetics",
author = "Kochetkova, {Tatiana V.} and Zayulina, {Kseniya S.} and Zhigarkov, {Vyacheslav S.} and Minaev, {Nikita V.} and Chichkov, {Boris N.} and Novikov, {Andrei A.} and Toshchakov, {Stepan V.} and Elcheninov, {Alexander G.} and Kublanov, {Ilya V.}",
note = "Funding information: This work of T. V. K., K. S. Z. and A. G. E. was supported by the Russian Foundation for Basic Research (grant number 17-04-01578). The Ministry of Science and Higher Education of Russia supported the work of IVK and SVT. N. V. M. was granted a Scholarship of the President of the Russian Federation SP-2728.2019.4. Chemotaxonomic studies (A. A. N.) were supported by the President of Russia (grant ???3801.2018.4; agreement 075-15-2019-1025).",
year = "2020",
month = feb,
doi = "10.1099/ijsem.0.003902",
language = "English",
volume = "70",
pages = "1192--1202",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Microbiology Society",
number = "2",

}

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TY - JOUR

T1 - Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14

AU - Kochetkova, Tatiana V.

AU - Zayulina, Kseniya S.

AU - Zhigarkov, Vyacheslav S.

AU - Minaev, Nikita V.

AU - Chichkov, Boris N.

AU - Novikov, Andrei A.

AU - Toshchakov, Stepan V.

AU - Elcheninov, Alexander G.

AU - Kublanov, Ilya V.

N1 - Funding information: This work of T. V. K., K. S. Z. and A. G. E. was supported by the Russian Foundation for Basic Research (grant number 17-04-01578). The Ministry of Science and Higher Education of Russia supported the work of IVK and SVT. N. V. M. was granted a Scholarship of the President of the Russian Federation SP-2728.2019.4. Chemotaxonomic studies (A. A. N.) were supported by the President of Russia (grant ???3801.2018.4; agreement 075-15-2019-1025).

PY - 2020/2

Y1 - 2020/2

N2 - A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).

AB - A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).

KW - Chloroflexi

KW - Chukotka hot spring

KW - Laser bioprinting

KW - LEMS

KW - Microbial dark matter

KW - Tepidiforma

KW - RNA, Ribosomal, 16S/genetics

KW - Phospholipids/chemistry

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Russia

KW - Chloroflexi/classification

KW - Hot Springs/microbiology

KW - Carotenoids/chemistry

KW - Arctic Regions

KW - Base Composition

KW - Bacterial Typing Techniques

KW - Fatty Acids/chemistry

KW - DNA, Bacterial/genetics

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U2 - 10.1099/ijsem.0.003902

DO - 10.1099/ijsem.0.003902

M3 - Article

C2 - 31769750

AN - SCOPUS:85081139572

VL - 70

SP - 1192

EP - 1202

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

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