TY - JOUR

T1 - Teburu—Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models

AU - Daneshgar, Assal

AU - Tang, Peter

AU - Remde, Christopher

AU - Lommel, Michael

AU - Moosburner, Simon

AU - Kertzscher, Ulrich

AU - Klein, Oliver

AU - Weinhart, Marie

AU - Pratschke, Johann

AU - Sauer, Igor M.

AU - Hillebrandt, Karl H.

N1 - Funding information: The authors thank Prof. Dr.-Ing. Klaus Affeld from the Biofluid Machanics Laboratory and the Institute for Imaging Science and Computational Modeling in Cardiovascular Medicine at Charité – Universitätsmedizin Berlin for his kind cooperation regarding Teburu's fluid dynamics and flow simulation. Furthermore, the authors thank the Einstein Center for Regenerative Therapies for funding this project.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.

AB - Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.

KW - 3D cell culture

KW - 3D-printing

KW - bioreactor

KW - optogenetics

KW - recellularization

UR - http://www.scopus.com/inward/record.url?scp=85069856159&partnerID=8YFLogxK

U2 - 10.1111/aor.13518

DO - 10.1111/aor.13518

M3 - Article

C2 - 31211867

AN - SCOPUS:85069856159

VL - 43

SP - 1035

EP - 1041

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 10

ER -