Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 279-280 |
| Fachzeitschrift | Bulletin of Insectology |
| Jahrgang | 64 |
| Ausgabenummer | SUPPL. 1 |
| Publikationsstatus | Veröffentlicht - 2011 |
| Extern publiziert | Ja |
Abstract
Xanthomonas spp. are Gram-negative bacteria with powerful molecular weapons to attack their plant hosts. Key for pathogenicity of Xanthomonas is a type III secretion system that injects a cocktail of effector proteins into plant cells to function as potent virulence factors. TAL (transcription activator-like) effectors from Xanthomonas function as transcriptional activators of plant genes in the plant nucleus. They contain a central domain of tandem, near-identical 34 amino-acid repeats. Each repeat recognizes a single base pair in a contiguous DNA sequence and two adjacent hypervariable amino acids per repeat specify the base that is bound. This modular DNA-binding code allows a simple reprogramming of DNA-binding specificity, a feature with high potential for biotechnology. We developed a method called "Golden TAL Technology" that allows a flexible assembly of TAL proteins with a designed order of repeats.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Insektenkunde
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in: Bulletin of Insectology, Jahrgang 64, Nr. SUPPL. 1, 2011, S. 279-280.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - TAL effectors from Xanthomonas: design of a programmable DNA-binding specificity
AU - Scholze, Heidi
AU - Streubel, Jana
AU - Boch, Jens
N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - Xanthomonas spp. are Gram-negative bacteria with powerful molecular weapons to attack their plant hosts. Key for pathogenicity of Xanthomonas is a type III secretion system that injects a cocktail of effector proteins into plant cells to function as potent virulence factors. TAL (transcription activator-like) effectors from Xanthomonas function as transcriptional activators of plant genes in the plant nucleus. They contain a central domain of tandem, near-identical 34 amino-acid repeats. Each repeat recognizes a single base pair in a contiguous DNA sequence and two adjacent hypervariable amino acids per repeat specify the base that is bound. This modular DNA-binding code allows a simple reprogramming of DNA-binding specificity, a feature with high potential for biotechnology. We developed a method called "Golden TAL Technology" that allows a flexible assembly of TAL proteins with a designed order of repeats.
AB - Xanthomonas spp. are Gram-negative bacteria with powerful molecular weapons to attack their plant hosts. Key for pathogenicity of Xanthomonas is a type III secretion system that injects a cocktail of effector proteins into plant cells to function as potent virulence factors. TAL (transcription activator-like) effectors from Xanthomonas function as transcriptional activators of plant genes in the plant nucleus. They contain a central domain of tandem, near-identical 34 amino-acid repeats. Each repeat recognizes a single base pair in a contiguous DNA sequence and two adjacent hypervariable amino acids per repeat specify the base that is bound. This modular DNA-binding code allows a simple reprogramming of DNA-binding specificity, a feature with high potential for biotechnology. We developed a method called "Golden TAL Technology" that allows a flexible assembly of TAL proteins with a designed order of repeats.
KW - AvrBs3
KW - Golden gate cloning
KW - TALE
KW - Transcription factor
KW - Zinc-finger proteins
UR - http://www.scopus.com/inward/record.url?scp=80054870973&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:80054870973
VL - 64
SP - 279
EP - 280
JO - Bulletin of Insectology
JF - Bulletin of Insectology
SN - 1721-8861
IS - SUPPL. 1
ER -