TY - JOUR

T1 - TAL effectors - pathogen strategies and plant resistance engineering

AU - Boch, Jens

AU - Bonas, Ulla

AU - Lahaye, Thomas

N1 - Publisher Copyright: © 2014 New Phytologist Trust. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2014/8/26

Y1 - 2014/8/26

N2 - Summary: Transcription activator-like effectors (TALEs) from plant pathogenic Xanthomonas spp. and the related RipTALs from Ralstonia solanacearum are DNA-binding proteins with a modular DNA-binding domain. This domain is both predictable and programmable, which simplifies elucidation of TALE function in planta and facilitates generation of DNA-binding modules with desired specificity for biotechnological approaches. Recently identified TALE host target genes that either promote or stop bacterial disease provide new insights into how expression of TALE genes affects the plant-pathogen interaction. Since its elucidation the TALE code has been continuously refined and now provides a mature tool that, in combination with transcriptome profiling, allows rapid isolation of novel TALE target genes. The TALE code is also the basis for synthetic promoter-traps that mediate recognition of TALE or RipTAL proteins in engineered plants. In this review, we will summarize recent findings in plant-focused TALE research. In addition, we will provide an outline of the newly established gene isolation approach for TALE or RipTAL host target genes with an emphasis on potential pitfalls.

AB - Summary: Transcription activator-like effectors (TALEs) from plant pathogenic Xanthomonas spp. and the related RipTALs from Ralstonia solanacearum are DNA-binding proteins with a modular DNA-binding domain. This domain is both predictable and programmable, which simplifies elucidation of TALE function in planta and facilitates generation of DNA-binding modules with desired specificity for biotechnological approaches. Recently identified TALE host target genes that either promote or stop bacterial disease provide new insights into how expression of TALE genes affects the plant-pathogen interaction. Since its elucidation the TALE code has been continuously refined and now provides a mature tool that, in combination with transcriptome profiling, allows rapid isolation of novel TALE target genes. The TALE code is also the basis for synthetic promoter-traps that mediate recognition of TALE or RipTAL proteins in engineered plants. In this review, we will summarize recent findings in plant-focused TALE research. In addition, we will provide an outline of the newly established gene isolation approach for TALE or RipTAL host target genes with an emphasis on potential pitfalls.

KW - Effector

KW - Genome editing

KW - Plant pathogen

KW - Resistance

KW - Susceptibility

KW - Transcription activator-like effector (TALE)

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=84911954031&partnerID=8YFLogxK

U2 - 10.1111/nph.13015

DO - 10.1111/nph.13015

M3 - Review article

C2 - 25539004

AN - SCOPUS:84911954031

VL - 204

SP - 823

EP - 832

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -